HAUSP Antibody (G-12): sc-377147
- HAUSP Antibody (G-12) is a mouse monoclonal IgG3 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 17-53 near the N-terminus of HAUSP of human origin
- recommended for detection of HAUSP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgGκ BP-HRP is the preferred secondary detection reagent for HAUSP Antibody (G-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with HAUSP Antibody (G-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
SEE ALSO...
HAUSP Antibody (G-12) is a mouse monoclonal IgG3 antibody that detects HAUSP (herpesvirus-associated ubiquitin-specific protease, USP7) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HAUSP plays a critical role in the regulation of p53, a key tumor suppressor protein, by deubiquitinating p53, which is essential for p53 stabilization and function. This interaction is particularly important because p53 is a crucial player in cellular responses to stress, DNA damage, and oncogenic signals, thereby influencing cell cycle regulation and apoptosis. HAUSP can stabilize p53 even in the presence of excess Mdm2, an E3 ubiquitin ligase that targets p53 for degradation, underscoring HAUSP′s significance in cancer biology and therapeutic strategies. HAUSP is predominantly localized in the nucleus, where HAUSP forms a micropunctate pattern and associates with ND10 nuclear structures, which are involved in various cellular processes, including the response to viral infections and stress. The gene encoding HAUSP is located on human chromosome band 16p13.2, highlighting HAUSP′s evolutionary conservation and importance across species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HAUSP Antibody (G-12) References:
- Loss of HAUSP-mediated deubiquitination contributes to DNA damage-induced destabilization of Hdmx and Hdm2. | Meulmeester, E., et al. 2005. Mol Cell. 18: 565-76. PMID: 15916963
- Molecular recognition of p53 and MDM2 by USP7/HAUSP. | Sheng, Y., et al. 2006. Nat Struct Mol Biol. 13: 285-91. PMID: 16474402
- The deubiquitinylation and localization of PTEN are regulated by a HAUSP-PML network. | Song, MS., et al. 2008. Nature. 455: 813-7. PMID: 18716620
- USP7/HAUSP stimulates repair of oxidative DNA lesions. | Khoronenkova, SV., et al. 2011. Nucleic Acids Res. 39: 2604-9. PMID: 21138959
- Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like domain and allosteric regulation by GMP-synthetase. | Faesen, AC., et al. 2011. Mol Cell. 44: 147-59. PMID: 21981925
- Nucleophosmin/B26 regulates PTEN through interaction with HAUSP in acute myeloid leukemia. | Noguera, NI., et al. 2013. Leukemia. 27: 1037-43. PMID: 23183427
- BCR-ABL disrupts PTEN nuclear-cytoplasmic shuttling through phosphorylation-dependent activation of HAUSP. | Morotti, A., et al. 2014. Leukemia. 28: 1326-33. PMID: 24317448
- DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. | Papp, SJ., et al. 2015. Elife. 4: PMID: 25756610
- The targetable role of herpes virus-associated ubiquitin-specific protease (HAUSP) in p190 BCR-ABL leukemia. | Carrà, G., et al. 2016. Oncol Lett. 12: 3123-3126. PMID: 27899971
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAUSP Antibody (G-12) | sc-377147 | 200 µg/ml | $316.00 | |||
HAUSP Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-552148 | 200 µg Ab; 40 µg BP | $354.00 | |||
HAUSP (G-12) Neutralizing Peptide | sc-377147 P | 100 µg/0.5 ml | $68.00 |