HAS3 Antibody (G-12): sc-365322

HAS3 Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects HAS3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). HAS3 (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HAS3, part of the hyaluronic acid synthase family, is crucial for synthesizing hyaluronic acid (HA), a vital component of the extracellular matrix that contributes to tissue hydration, elasticity, and cellular signaling. Unlike HAS1 and HAS2, which produce high molecular weight HA, HAS3 generates lower molecular weight HA, which plays distinct roles in cellular processes. HAS3 expression is particularly important in the epidermis, where interferon-gamma significantly upregulates and transforming growth factor-beta downregulates HAS3, indicating involvement in inflammatory responses and tissue repair mechanisms. Human HAS3 gene is located on chromosome 16q22.1, and expression is more pronounced in actively growing cells, highlighting an essential role in development and cellular dynamics.

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HAS3 Antibody (G-12) References:

1. Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties.  |  Itano, N., et al. 1999. J Biol Chem. 274: 25085-92. PMID: 10455188
2. Expression of human hyaluronan synthases in response to external stimuli.  |  Jacobson, A., et al. 2000. Biochem J. 348 Pt 1: 29-35. PMID: 10794710
3. Regulation of hyaluronan synthase gene expression in human periodontal ligament cells by tumour necrosis factor-alpha, interleukin-1beta and interferon-gamma.  |  Ijuin, C., et al. 2001. Arch Oral Biol. 46: 767-72. PMID: 11389868
4. Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration.  |  Itano, N., et al. 2002. Proc Natl Acad Sci U S A. 99: 3609-14. PMID: 11891291
5. Identification and analysis of the promoter region of the human HAS3 gene.  |  Wang, S., et al. 2015. Biochem Biophys Res Commun. 460: 1008-14. PMID: 25843802
6. HAS3 underexpression as an indicator of poor prognosis in patients with urothelial carcinoma of the upper urinary tract and urinary bladder.  |  Chang, IW., et al. 2015. Tumour Biol. 36: 5441-50. PMID: 25934334
7. HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.  |  Arasu, UT., et al. 2020. Cell Mol Life Sci. 77: 4093-4115. PMID: 31820036
8. Targeting hyaluronic acid synthase-3 (HAS3) for the treatment of advanced renal cell carcinoma.  |  Wang, J., et al. 2022. Cancer Cell Int. 22: 421. PMID: 36581895
9. Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis.  |  Luo, X., et al. 2024. J Pineal Res. 76: e12940. PMID: 38402581
10. Chromosomal localization of the human and mouse hyaluronan synthase genes.  |  Spicer, AP., et al. 1997. Genomics. 41: 493-7. PMID: 9169154

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HAS3 Antibody (G-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HAS3 Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HAS3 Antibody (G-12) Specifications

Alternate Product Name: Hyaluronan Synthase 3

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 124-187

Epitope Target: HAS3

Epitope Species: human

Product Reactivity/Detection: HAS3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HAS3 Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin