Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HAS2 Double Nickase Plasmid (m): sc-420798-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAS2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HAS2 Double Nickase Plasmid (m) and HAS2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Has2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HAS2 Antibody (A-7): sc-514737
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAS2 Double Nickase Plasmid (m) | sc-420798-NIC | 20 µg | $410.00 | |||
HAS2 Double Nickase Plasmid (m2) | sc-420798-NIC-2 | 20 µg | $410.00 |
Mouse Has2 encodes hyaluronan synthase 2 (HAS2), a membrane-associated glycosyltransferase that polymerizes hyaluronan at the plasma membrane and exports it into the extracellular matrix. HAS2-driven hyaluronan production regulates pericellular matrix assembly, tissue hydration, and cell–matrix signaling that influences adhesion, migration, and proliferation through pathways involving CD44 and RHAMM as well as cytoskeletal remodeling. Has2 activity is tightly linked to developmental morphogenesis, wound repair, inflammatory signaling, and fibroblast activation, with altered hyaluronan homeostasis implicated in fibrosis, vascular remodeling, and tumor-associated stromal biology. As a central determinant of extracellular matrix mechanics and growth factor availability, Has2 is frequently studied in contexts of epithelial–mesenchymal transition, angiogenesis, and immune cell trafficking.
HAS2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Has2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Has2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Has2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Has2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.