Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HAS2 Double Nickase Plasmid (h): sc-401032-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAS2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HAS2 Double Nickase Plasmid (h) and HAS2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HAS2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HAS2 Antibody (A-7): sc-514737
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAS2 Double Nickase Plasmid (h) | sc-401032-NIC | 20 µg | $410.00 | |||
HAS2 Double Nickase Plasmid (h2) | sc-401032-NIC-2 | 20 µg | $410.00 |
Human HAS2 encodes hyaluronan synthase 2, a membrane-associated enzyme that polymerizes UDP-glucuronic acid and UDP-N-acetylglucosamine to produce high–molecular weight hyaluronan at the plasma membrane. HAS2-driven hyaluronan deposition shapes the extracellular matrix and pericellular coat, influencing cell hydration, adhesion, and migration through interactions with receptors such as CD44 and RHAMM. Its activity is integrated with pathways controlling tissue remodeling and inflammatory signaling, and is regulated by growth factor cues and cellular metabolic state. Dysregulated HAS2 expression or hyaluronan accumulation is frequently studied in contexts of fibrosis, chronic inflammation, and tumor microenvironment biology.
HAS2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HAS2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HAS2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HAS2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HAS2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.