Haptoglobin α Antibody (C-8): sc-376893

Haptoglobin α Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Haptoglobin α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Haptoglobin α (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Haptoglobin, a crucial blood plasma protein, plays a vital role in binding free hemoglobin released from erythrocytes, thereby preventing oxidative damage and sequestering iron to inhibit bacterial growth that could exploit hemolysis. This protective mechanism is particularly important during inflammatory responses, as elevated Haptoglobin levels can indicate inflammation and assist in the clearance of the Haptoglobin-hemoglobin complex by the reticulo-endothelial system. Structurally, Haptoglobin is composed of two alpha and two beta chains linked by disulfide bridges, resulting from the cleavage of a common precursor protein during synthesis. In humans, Haptoglobin exists in two allelic forms, Haptoglobin 1 (Hp1) and Haptoglobin 2 (Hp2), with Hp2 arising from a partial gene duplication of Hp1. The three known phenotypes—Hp1-1, Hp2-1, and Hp2-2—are associated with various health conditions, including diabetes, cardiovascular diseases, and increased susceptibility to Parkinson′s and Crohn′s diseases. Additionally, Haptoglobin levels serve as a valuable diagnostic marker for hemolytic anemia, reflecting the abnormal breakdown of red blood cells, and are expressed in mammalian hepatocytes as well as in other tissues such as skin, lung, and kidney.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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Haptoglobin α Antibody (C-8) References:

1. Haptoglobin-alpha subunit as potential serum biomarker in ovarian cancer: identification and characterization using proteomic profiling and mass spectrometry.  |  Ye, B., et al. 2003. Clin Cancer Res. 9: 2904-11. PMID: 12912935
2. Pretreatment serum levels of C-reactive protein, alpha 1-antitrypsin, haptoglobin, alpha 1-acid glycoprotein and tissue polypeptide antigen in cervical carcinoma.  |  Avall Lundqvist, E., et al. 1991. Eur J Gynaecol Oncol. 12: 375-83. PMID: 1717278
3. Identification of haptoglobin alpha-2FF variants in mid-trimester maternal serum as potential markers for Down syndrome.  |  Myrick, JE., et al. 1990. Appl Theor Electrophor. 1: 233-41. PMID: 2151560
4. Proteomic and genomic biomarkers for Non-Small Cell Lung Cancer: Peroxiredoxin, Haptoglobin, and Alpha-1 antitrypsin.  |  Najafi, Z., et al. 2020. Cancer Med. 9: 3974-3982. PMID: 32232956
5. Clinical Interest of Serum Alpha-2 Macroglobulin, Apolipoprotein A1, and Haptoglobin in Patients with Non-Alcoholic Fatty Liver Disease, with and without Type 2 Diabetes, before or during COVID-19.  |  Deckmyn, O., et al. 2022. Biomedicines. 10: PMID: 35327501
6. Heterogeneity of human haptoglobin alpha chains detected by two-dimensional gel electrophoresis.  |  John, HA. and Purdom, IF. 1987. Genet Res. 50: 17-21. PMID: 3653685
7. Gene action in the human haptoglobins. IV. Amino acid sequence studies on the haptoglobin alpha chains.  |  Black, JA. and Dixon, GH. 1970. Can J Biochem. 48: 133-46. PMID: 5512536
8. Possible heterogeneity in the phosphoglycolate phosphatase (PGP)-haptoglobin alpha (HPA) linkage.  |  Bale, SJ., et al. 1984. Am J Hum Genet. 36: 808-14. PMID: 6089552
9. Characterization of human haptoglobin cDNAs coding for alpha 2FS beta and alpha 1S beta variants.  |  van der Straten, A., et al. 1984. FEBS Lett. 168: 103-7. PMID: 6546723
10. Glycosylation of alpha-1-proteinase inhibitor and haptoglobin in ovarian cancer: evidence for two different mechanisms.  |  Turner, GA., et al. 1995. Glycoconj J. 12: 211-8. PMID: 7496134

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Haptoglobin α Antibody (C-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Haptoglobin α Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Haptoglobin α Antibody (C-8) Specifications

Alternate Product Name: Haptoglobin α

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-100

Epitope Target: Haptoglobin

Epitope Species: mouse

Product Reactivity/Detection: Haptoglobin α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Haptoglobin α Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Haptoglobin α antibody is Haptoglobin α Antibody (B-2): sc-365396.