Haptoglobin α Antibody (B-2): sc-365396

Haptoglobin α Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Haptoglobin alpha protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Haptoglobin α (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Haptoglobin (Hp) is a crucial blood plasma protein that plays a vital role in binding free hemoglobin released from erythrocytes, thereby preventing oxidative damage and sequestering iron to inhibit bacterial growth during hemolysis. This protective function is particularly important in inflammatory responses, as elevated Haptoglobin levels can indicate inflammation and tissue damage. The Haptoglobin-Hemoglobin complex is subsequently cleared by the reticulo-endothelial system, highlighting Hp′s role in maintaining homeostasis. Structurally, Haptoglobin is composed of two α and two β chains linked by disulfide bridges, resulting from the cleavage of a common precursor protein during synthesis. In humans, Haptoglobin exists in two allelic forms, Haptoglobin 1 (Hp1) and Haptoglobin 2 (Hp2), with Hp2 arising from a partial gene duplication of Hp1. The three known phenotypes—Hp1-1, Hp2-1, and Hp2-2—are associated with various health conditions, including diabetes, cardiovascular diseases, and susceptibility to neurodegenerative disorders like Parkinson′s disease and inflammatory conditions such as Crohn′s disease. Additionally, Haptoglobin levels are clinically significant for diagnosing hemolytic anemia, a condition characterized by the abnormal breakdown of red blood cells, and Haptoglobin is expressed in mammalian hepatocytes as well as in other tissues, including skin, lung, and kidney.

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Haptoglobin α Antibody (B-2) References:

1. Haptoglobin alpha-subunit and hepatocyte growth factor can potentially serve as serum tumor biomarkers in small cell lung cancer.  |  Bharti, A., et al. 2004. Anticancer Res. 24: 1031-8. PMID: 15154618
2. Binding of acellular, native and cross-linked human hemoglobins to haptoglobin: enhanced distribution and clearance in the rat.  |  Ship, NJ., et al. 2005. Am J Physiol Gastrointest Liver Physiol. 288: G1301-9. PMID: 15890714
3. Haptoglobin expression by shed endometrial tissue fragments found in peritoneal fluid.  |  Sharpe-Timms, KL. 2005. Fertil Steril. 84: 22-30. PMID: 16009149
4. The acute phase protein haptoglobin is a mammalian extracellular chaperone with an action similar to clusterin.  |  Yerbury, JJ., et al. 2005. Biochemistry. 44: 10914-25. PMID: 16086594
5. Effect of sucrose on inflammatory markers in overweight humans.  |  Sørensen, LB., et al. 2005. Am J Clin Nutr. 82: 421-7. PMID: 16087988
6. Serum free hemoglobin concentrations in healthy individuals are related to haptoglobin type.  |  Na, N., et al. 2005. Clin Chem. 51: 1754-5. PMID: 16120964
7. Haptoglobin polymorphism predicts 30-day mortality and heart failure in patients with diabetes and acute myocardial infarction.  |  Suleiman, M., et al. 2005. Diabetes. 54: 2802-6. PMID: 16123372
8. Haptoglobin phenotype, sleep-disordered breathing, and the prevalence of cardiovascular disease: the Sleep Heart Health Study.  |  Levy, AP., et al. 2005. Sleep. 28: 207-13. PMID: 16171245
9. C/EBP alpha and C/EBP beta regulate haptoglobin gene expression during rat liver development and the acute-phase response.  |  Dinić, S., et al. 2005. Mol Biol Rep. 32: 141-7. PMID: 16172914
10. Acute-phase related binding ability of p53 for the hormone response element of the haptoglobin gene in adult rats.  |  Mihailović, M., et al. 2005. Cell Biol Int. 29: 968-70. PMID: 16199185
11. Proteomic identification of fucosylated haptoglobin alpha isoforms in ascitic fluids and its localization in ovarian carcinoma tissues from Mexican patients.  |  Garibay-Cerdenares, OL., et al. 2014. J Ovarian Res. 7: 27. PMID: 24576319
12. Urinary haptoglobin, alpha-1 anti-chymotrypsin and retinol binding protein identified by proteomics as potential biomarkers for lupus nephritis.  |  Aggarwal, A., et al. 2017. Clin Exp Immunol. 188: 254-262. PMID: 28120479

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Haptoglobin α Antibody (B-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Haptoglobin α Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Haptoglobin α Antibody (B-2) Specifications

Alternate Product Name: Haptoglobin

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-150

Epitope Target: Haptoglobin

Epitope Species: human

Product Reactivity/Detection: Haptoglobin α and Haptoglobin-related protein

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Haptoglobin α Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin