HAPLN3 Antibody (F-3): sc-377480
- HAPLN3 Antibody (F-3) is a mouse monoclonal IgG1 κ HAPLN3 antibody provided at 200 µg/ml
- raised against amino acids 74-118 mapping within an internal region of HAPLN3 of human origin
- HAPLN3 Antibody (F-3) is recommended for detection of HAPLN3 of human origin by WB, IP, IF and ELISA
- Anti-HAPLN3 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for HAPLN3 Antibody (F-3) for WB applications. This reagent is now offered in a bundle with HAPLN3 Antibody (F-3) (see ordering information below).
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HAPLN3 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects HAPLN3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HAPLN3 Antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HAPLN3, or Hyaluronan and proteoglycan link protein 3, is a 360 amino acid protein encoded by the human gene HAPLN3 and is a member of the HAPLN family, characterized by one immunoglobulin-like V-type domain and two Link domains. HAPLN3 plays a crucial role in mediating the binding of hyaluronic acid-containing complexes, which is essential for the formation of the hyaluronan-associated matrix in the central nervous system (CNS). This matrix is vital for neuronal conduction and structural stabilization, highlighting HAPLN3′s importance in maintaining the integrity of neural tissues. HAPLN3 is implicated in the formation of extracellular matrices that contribute to perineuronal nets, which are essential for neuronal function and protection. HAPLN3 expression is widespread, with the highest levels found in the spleen and placenta, indicating a significant role in various biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HAPLN3 Antibody (F-3) References:
- Formation of hyaluronan- and versican-rich pericellular matrix is required for proliferation and migration of vascular smooth muscle cells. | Evanko, SP., et al. 1999. Arterioscler Thromb Vasc Biol. 19: 1004-13. PMID: 10195929
- A hyaluronan binding link protein gene family whose members are physically linked adjacent to chondroitin sulfate proteoglycan core protein genes: the missing links. | Spicer, AP., et al. 2003. J Biol Chem. 278: 21083-91. PMID: 12663660
- The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. | Clark, HF., et al. 2003. Genome Res. 13: 2265-70. PMID: 12975309
- Molecular cloning of Bral2, a novel brain-specific link protein, and immunohistochemical colocalization with brevican in perineuronal nets. | Bekku, Y., et al. 2003. Mol Cell Neurosci. 24: 148-59. PMID: 14550776
- Lp3/Hapln3, a novel link protein that co-localizes with versican and is coordinately up-regulated by platelet-derived growth factor in arterial smooth muscle cells. | Ogawa, H., et al. 2004. Matrix Biol. 23: 287-98. PMID: 15464361
- Composition of perineuronal nets in the adult rat cerebellum and the cellular origin of their components. | Carulli, D., et al. 2006. J Comp Neurol. 494: 559-77. PMID: 16374793
- Formation of hyaluronan- and versican-rich pericellular matrix by prostate cancer cells promotes cell motility. | Ricciardelli, C., et al. 2007. J Biol Chem. 282: 10814-25. PMID: 17293599
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAPLN3 Antibody (F-3) | sc-377480 | 200 µg/ml | $322.00 | |||
HAPLN3 Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-526101 | 200 µg Ab; 10 µg BP | $361.00 | |||
HAPLN3 Antibody (F-3) AC | sc-377480 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HAPLN3 Antibody (F-3) HRP | sc-377480 HRP | 200 µg/ml | $322.00 | |||
HAPLN3 Antibody (F-3) FITC | sc-377480 FITC | 200 µg/ml | $336.00 | |||
HAPLN3 Antibody (F-3) PE | sc-377480 PE | 200 µg/ml | $349.00 | |||
HAPLN3 Antibody (F-3) Alexa Fluor® 488 | sc-377480 AF488 | 200 µg/ml | $364.00 | |||
HAPLN3 Antibody (F-3) Alexa Fluor® 546 | sc-377480 AF546 | 200 µg/ml | $364.00 | |||
HAPLN3 Antibody (F-3) Alexa Fluor® 594 | sc-377480 AF594 | 200 µg/ml | $364.00 | |||
HAPLN3 Antibody (F-3) Alexa Fluor® 647 | sc-377480 AF647 | 200 µg/ml | $364.00 | |||
HAPLN3 Antibody (F-3) Alexa Fluor® 680 | sc-377480 AF680 | 200 µg/ml | $364.00 | |||
HAPLN3 Antibody (F-3) Alexa Fluor® 790 | sc-377480 AF790 | 200 µg/ml | $364.00 |