HAO2 CRISPR/Cas9 KO Plasmid (h): sc-405855
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAO2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HAO2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAO2 CRISPR/Cas9 KO Plasmid (h) | sc-405855 | 20 µg | $397.00 |
Overview
HAO2 (hydroxyacid oxidase 2) encodes a peroxisomal FMN-dependent oxidase involved in the oxidation of 2-hydroxy fatty acids, contributing to lipid catabolism and peroxisome-mediated redox homeostasis. Through generation of hydrogen peroxide during substrate oxidation, HAO2 intersects with cellular oxidative stress control and metabolic adaptation pathways linked to peroxisomal function. Altered HAO2 expression has been associated with metabolic reprogramming in liver biology and reported in multiple transcriptomic studies of hepatocellular carcinoma, supporting its use as a marker for investigating tumor-associated metabolic states. Disruption or modulation of HAO2 is therefore useful for probing peroxisomal lipid metabolism, reactive oxygen species handling, and downstream signaling consequences in human cell models.
HAO2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HAO2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HAO2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HAO2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HAO2 protein expression.
This CRISPR knockout system enables efficient generation of HAO2-deficient cell models for investigation of HAO2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HAO2 exon(s) critical for HAO2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HAO2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HAO2 CRISPR/Cas9 KO Plasmid (h) and HAO2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HAO2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HAO2 HDR Plasmid (h) and HAO2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HAO2 homology arms to support homology-directed repair at defined HAO2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.