Hairy Antibody (1/24): sc-53297
- Hairy Antibody (1/24) is a mouse monoclonal IgG1 κ Hairy antibody, cited in 1 publications, provided at 200 µg/ml
- raised against Hairy of Drosophila origin
- Hairy Antibody (1/24) is recommended for detection of Hairy of d origin by WB
- Anti-Hairy Antibody (1/24) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Hairy Antibody (1/24) for WB applications. These reagents are now offered in bundles with Hairy Antibody (1/24) (see ordering information below).
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Hairy Antibody (1/24) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hairy protein of Drosophila melanogaster origin by western blotting (WB). Anti-Hairy antibody (1/24) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hairy protein, a 377-amino acid pair-rule protein, plays a crucial role in embryonic segmentation and adult bristle patterning by repressing transcription. This transcriptional repression ensures correct spatial and temporal expression of target genes during embryogenesis. Hairy (1/24) antibody achieves function by forming a complex with corepressors, which is essential for activity. Hairy protein localizes to nuclei of cells in eight distinct regions of early embryo, allowing precise regulation of gene expression in these critical developmental stages. Hairy protein is not expressed in embryonic nervous system, highlighting specific role in segmentation rather than neural development. Hairy protein contains a domain similar to proto-oncogene N-myc, which may facilitate protein dimerization and/or DNA binding, further underscoring importance in gene regulation. Hairy protein plays a role in repressing normal spatial expression of Fushi tarazu, another pair-rule protein, thereby contributing to intricate network of gene interactions that govern Drosophila development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hairy Antibody (1/24) References:
- Opposing inputs by Hedgehog and Brinker define a stripe of hairy expression in the Drosophila leg imaginal disc. | Kwon, C., et al. 2004. Development. 131: 2681-92. PMID: 15128656
- Hairy transcriptional repression targets and cofactor recruitment in Drosophila. | Bianchi-Frias, D., et al. 2004. PLoS Biol. 2: E178. PMID: 15252443
- Point mutations in the Drosophila hairy gene demonstrate in vivo requirements for basic, helix-loop-helix, and WRPW domains. | Wainwright, SM. and Ish-Horowicz, D. 1992. Mol Cell Biol. 12: 2475-83. PMID: 1588951
- Delta and Hairy establish a periodic prepattern that positions sensory bristles in Drosophila legs. | Joshi, M., et al. 2006. Dev Biol. 293: 64-76. PMID: 16542648
- hairy gene function in the Drosophila eye: normal expression is dispensable but ectopic expression alters cell fates. | Brown, NL., et al. 1991. Development. 113: 1245-56. PMID: 1811940
- The Drosophila hairy protein acts in both segmentation and bristle patterning and shows homology to N-myc. | Rushlow, CA., et al. 1989. EMBO J. 8: 3095-103. PMID: 2479541
- Spatial control of hairy protein expression during embryogenesis. | Hooper, KL., et al. 1989. Development. 107: 489-504. PMID: 2612375
- Expression, function, and regulation of the hairy segmentation protein in the Drosophila embryo. | Carroll, SB., et al. 1988. Genes Dev. 2: 883-90. PMID: 3209072
- Drosophila CtBP: a Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression. | Poortinga, G., et al. 1998. EMBO J. 17: 2067-78. PMID: 9524128
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hairy Antibody (1/24) | sc-53297 | 200 µg/ml | $322.00 | |||
Hairy Antibody (1/24): m-IgG Fc BP-HRP Bundle | sc-526789 | 200 µg Ab; 10 µg BP | $361.00 | |||
Hairy Antibody (1/24): m-IgG1 BP-HRP Bundle | sc-532162 | 200 µg Ab; 20 µg BP | $361.00 | |||
Hairy Antibody (1/24) AC | sc-53297 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Hairy Antibody (1/24) HRP | sc-53297 HRP | 200 µg/ml | $322.00 | |||
Hairy Antibody (1/24) FITC | sc-53297 FITC | 200 µg/ml | $336.00 | |||
Hairy Antibody (1/24) PE | sc-53297 PE | 200 µg/ml | $349.00 | |||
Hairy Antibody (1/24) Alexa Fluor® 488 | sc-53297 AF488 | 200 µg/ml | $364.00 | |||
Hairy Antibody (1/24) Alexa Fluor® 546 | sc-53297 AF546 | 200 µg/ml | $364.00 | |||
Hairy Antibody (1/24) Alexa Fluor® 594 | sc-53297 AF594 | 200 µg/ml | $364.00 | |||
Hairy Antibody (1/24) Alexa Fluor® 647 | sc-53297 AF647 | 200 µg/ml | $364.00 | |||
Hairy Antibody (1/24) Alexa Fluor® 680 | sc-53297 AF680 | 200 µg/ml | $364.00 | |||
Hairy Antibody (1/24) Alexa Fluor® 790 | sc-53297 AF790 | 200 µg/ml | $364.00 |