Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HADHA CRISPR/Cas9 KO Plasmid (m): sc-430176
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HADHA CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HADHA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HADHA Antibody (E-8): sc-374497
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HADHA CRISPR/Cas9 KO Plasmid (m) | sc-430176 | 20 µg | $397.00 |
Overview
Mouse Hadha encodes the alpha subunit of the mitochondrial trifunctional protein (HADHA), a key enzyme complex in long-chain fatty acid β-oxidation that catalyzes enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activities. HADHA supports mitochondrial energy metabolism by coupling lipid catabolism to acetyl-CoA production and downstream TCA cycle flux, with broad impacts on redox balance and metabolic adaptation. Disruption of HADHA function is linked to impaired mitochondrial fatty acid utilization and is relevant to inborn errors of metabolism affecting oxidative tissues such as liver, heart, and skeletal muscle. Hadha is therefore widely studied in pathways intersecting mitochondrial homeostasis, nutrient stress responses, and lipid-driven signaling.
HADHA CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hadha gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hadha together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hadha open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HADHA protein expression.
This CRISPR knockout system enables efficient generation of Hadha-deficient cell models for investigation of HADHA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hadha exon(s) critical for HADHA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hadha genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HADHA CRISPR/Cas9 KO Plasmid (m) and HADHA CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hadha locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HADHA HDR Plasmid (m) and HADHA HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hadha homology arms to support homology-directed repair at defined Hadha target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.