H6PD Antibody (C-10): sc-377180

H6PD Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects H6PD protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-H6PD antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. H6PD protein, also known as hexose-6-phosphate dehydrogenase, is a crucial enzyme composed of 789 amino acids, encoded by the human gene H6PD. H6PD plays a vital role in cellular metabolism by catalyzing the conversion of glucose-6-phosphate to 6-phosphogluconolactone within the endoplasmic reticulum, a process that generates reduced nicotinamide adenine dinucleotide phosphate (NADPH). This cofactor is essential for the activity of 11β-hydroxysteroid dehydrogenase type 1, which is responsible for converting inactive cortisone into active hormone cortisol, thereby influencing various physiological processes, including stress response and metabolism. The unique structural features of H6PD, with its N-terminal section belonging to the glucose-6-phosphate dehydrogenase family and the C-terminal section to the glucosamine/galactosamine-6-phosphate isomerase family, underscore its bifunctional nature and highlight its importance in maintaining cellular redox balance and steroid hormone regulation.

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H6PD Antibody (C-10) References:

1. Mutations in the genes encoding 11beta-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase interact to cause cortisone reductase deficiency.  |  Draper, N., et al. 2003. Nat Genet. 34: 434-9. PMID: 12858176
2. A study of the hexose-6-phosphate dehydrogenase gene R453Q and 11beta-hydroxysteroid dehydrogenase type 1 gene 83557insA polymorphisms in the polycystic ovary syndrome.  |  San Millán, JL., et al. 2005. J Clin Endocrinol Metab. 90: 4157-62. PMID: 15827106
3. Genotypes at 11beta-hydroxysteroid dehydrogenase type 11B1 and hexose-6-phosphate dehydrogenase loci are not risk factors for apparent cortisone reductase deficiency in a large population-based sample.  |  White, PC. 2005. J Clin Endocrinol Metab. 90: 5880-3. PMID: 16091483
4. Hexose-6-phosphate dehydrogenase knock-out mice lack 11 beta-hydroxysteroid dehydrogenase type 1-mediated glucocorticoid generation.  |  Lavery, GG., et al. 2006. J Biol Chem. 281: 6546-51. PMID: 16356929
5. Why is 11beta-hydroxysteroid dehydrogenase type 1 facing the endoplasmic reticulum lumen? Physiological relevance of the membrane topology of 11beta-HSD1.  |  Odermatt, A., et al. 2006. Mol Cell Endocrinol. 248: 15-23. PMID: 16412558
6. Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase- 6-phosphogluconolactonase.  |  Crooke, A., et al. 2006. FEBS J. 273: 1537-46. PMID: 16689939
7. Hexose 6-phosphate dehydrogenase (H6PD) and corticosteroid metabolism.  |  White, PC., et al. 2007. Mol Cell Endocrinol. 265-266: 89-92. PMID: 17240046
8. Properties of a chimeric glucose dehydrogenase improved by site directed mutagenesis.  |  Tripura, C. and Podile, AR. 2007. J Biotechnol. 131: 197-204. PMID: 17669536
9. Mutations of the hexose-6-phosphate dehydrogenase gene rarely cause hyperandrogenemic polycystic ovary syndrome.  |  Qin, K. and Rosenfield, RL. 2011. Steroids. 76: 135-9. PMID: 21050867
10. The R453Q and D151A polymorphisms of hexose-6-phosphate dehydrogenase gene (H6PD) influence the polycystic ovary syndrome (PCOS) and obesity.  |  Martínez-García, MA., et al. 2012. Gene. 497: 38-44. PMID: 22306327
11. Hexose-6-phosphate dehydrogenase controls cancer cell proliferation and migration through pleiotropic effects on the unfolded-protein response, calcium homeostasis, and redox balance.  |  Tsachaki, M., et al. 2018. FASEB J. 32: 2690-2705. PMID: 29295867
12. Hexose-6-phosphate dehydrogenase blockade reverses prostate cancer drug resistance in xenograft models by glucocorticoid inactivation.  |  Li, J., et al. 2021. Sci Transl Med. 13: PMID: 34039740

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-H6PD Antibody (C-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-H6PD Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

H6PD Antibody (C-10) Specifications

Alternate Product Name: H6PD

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 501-791

Epitope Target: H6PD

Epitope Species: human

Product Reactivity/Detection: H6PD

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for H6PD Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin