H2-I/E kappa Antibody (5K42) recommended for detection of H2-I/Eκ alloantigen on cells of the H-2r haplotype of mouse origin by FCM

H2-I/Eκ Antibody (5K42): sc-71204

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H2-I/Eκ Antibody (5K42) is rated 2.0 out of 5 by 1.
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Datasheets

    • H2-I/Eκ Antibody (5K42) is a mouse monoclonal IgG2a provided at 100 µg/ml
    • raised against C3H skin graft and splenocytes of mouse origin
    • recommended for detection of H2-I/Eκ alloantigen on cells of the H-2r haplotype of mouse origin by FCM; non cross-reactive with H2-d and H2-p haplotypes
    • available conjugated to phycoerythrin for IF, IHC(P) and FCM

If I want to use H2-I/Eκ (5K42): sc-71204 antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: Cweed
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-24
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