Anti-H2-I-A/I-E Antibody (M5/114) is a rat monoclonal IgG2bH2-I-A/I-E antibody provided at 200 µg/ml
raised against activated C57BL/6 mouse spleen cells
H2-I-A/I-E Antibody (M5/114) is recommended for detection of I-Ab, I-Ad, I-Aq, I-Ed and I-Ek MHC class II alloantigens of mouse origin by IF and FCM; non cross-reactive with I-Af, I-Ak or I-As; detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes, but not mice carrying the H-2s or H-2f
Anti-H2-I-A/I-E Antibody (M5/114) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of H2-I-A/I-E (M5/114): sc-23940.
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possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
H2-I-A/I-E Antibody (M5/114) is a high quality monoclonal H2-I-A/I-E antibody (also designated H2-I-A/I-E antibody) suitable for the detection of the H2-I-A/I-E protein of mouse origin. H2-I-A/I-E Antibody (M5/114) is available as both the non-conjugated anti-H2-I-A/I-E antibody form, as well as multiple conjugated forms of anti-H2-I-A/I-E antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Major histocompatibility complex (MHC) molecules, which include human leu-kocyte antigens (HLAs), form an integral part of the immune response system. They are cell-surface receptors that bind foreign peptides and present them to cytotoxic T lymphocytes (CTLs). The differential structural properties of MHC class I and II molecules account for their respective roles in activating different populations of T lymphocytes. The M5/114 (M5/114.15.2) monoclonal antibody reacts with the mouse MHC class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed and I-Ek; not I-Af, I-Ak or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u, but not from mice carrying the H-2s or H-2f haplotypes. The M5/ 114 (M5/114.15.2) mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q and H-2u, but not H-2f, H-2k or H-2s haplotypes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
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