H2-I-A/I-E Antibody (M5/114.15.2) recommended for detection of I-Ab, I-Ad, I-Aq, I-Ed and I-Ek MHC class II alloantigens of mouse origin by IF and FCM

H2-I-A/I-E Antibody (M5/114.15.2): sc-81730

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H2-I-A/I-E Antibody (M5/114.15.2) is rated 2.0 out of 5 by 1.
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Datasheets

    • H2-I-A/I-E Antibody (M5/114.15.2) is a rat monoclonal IgG2b provided at 200 µg/ml
    • raised against activated C57BL/6 mouse spleen cells
    • recommended for detection of I-Ab, I-Ad, I-Aq, I-Ed and I-Ek MHC class II alloantigens of mouse origin by IF and FCM; non cross-reactive with I-Af, I-Ak or I-As; detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes, but not mice carrying the H-2s or H-2f
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM

How can H2-I-A/I-E (M5/114.15.2): sc-81730 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-81730 PE) or fluoroscein (sc-81730 FITC).
Answered by: Technical Support
Date published: 2017-03-01
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