H2-I/A Antibody (NIMR-4) recommended for detection of non-polymorphic I-A-encoded epitope on MHC class II antigens of mouse origin by IF and FCM

H2-I/A Antibody (NIMR-4): sc-52548

H2-I/A Antibody (NIMR-4) is rated 4.0 out of 5 by 2.
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Datasheets

    • H2-I/A Antibody (NIMR-4) is a rat monoclonal IgG2b provided at 200 µg/ml
    • raised against B-lymphocytes of mouse origin
    • H2-I/A Antibody (NIMR-4) is recommended for detection of non-polymorphic I-A-encoded epitope on MHC class II antigens of mouse origin by IF and FCM
    • Anti-H2-I/A Antibody (NIMR-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of H2-I/A (NIMR-4): sc-52548.

How can H2-I/A (NIMR-4): sc-52548 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-52548 PE) or fluoroscein (sc-52548 FITC).
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from IF (pfa) publishable data with mouse brain IF (pfa) publishable data with mouse brain - SCBT Publication Review
Date published: 2015-02-06
Rated 4 out of 5 by from Produced robust indirect FCM data for mouse Produced robust indirect FCM data for mouse peripheral blood leukocytes stained with H2-I/A . -SCBT QC
Date published: 2013-11-26
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