H2-Db Antibody (5K45) recommended for detection of α3 domain of H2-Db class I MHC antigen of mouse origin by IF and FCM

H2-Db Antibody (5K45): sc-71199

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Datasheets

    • H2-Db Antibody (5K45) is a mouse monoclonal IgG2a provided at 100 µg/ml
    • raised against C3H.SW splenocytes of mouse origin
    • recommended for detection of α3 domain of H2-Db class I MHC antigen of mouse origin by IF and FCM; may cross-react with the α3 domain of H2-Ld and with H2-Dq and H2-Lq; non cross-reactive with H2-Kd or H2-Dd
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM

If I want to use H2-Db (5K45): sc-71199 for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: Germaine
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-24
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