H2-D^b Antibody (27-11-13): sc-52540

H2-Db Antibody (27-11-13) is a mouse monoclonal IgG2a antibody that detects H2-Db, a class I major histocompatibility complex (MHC) antigen of mouse origin, by immunofluorescence (IF) and flow cytometry (FCM). H2-Db (27-11-13) antibody is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. The H2 gene complex encodes MHC Class I molecules, which are crucial for the immune response as they present endogenously synthesized peptides to CD8+ T lymphocytes, typically cytotoxic T cells. The structure of H2-Db consists of a highly polymorphic alpha chain that forms a heterodimer with the invariant beta-2 microglobulin, allowing for a diverse range of peptide presentation. This structural diversity is essential for the immune system′s ability to recognize and respond to a wide array of pathogens. H2-Db expression on most nucleated cells varies by cell type, influencing the activation or inhibition of natural killer (NK) cells and playing a critical role in the positive and negative selection of CD8+ T cells during thymic development. Understanding H2-Db function and structure is vital for elucidating its role in immune regulation and potential implications in immunotherapy and transplantation.

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H2-D^b Antibody (27-11-13) References:

1. The binary logic of antigen processing and presentation to T cells.  |  Yewdell, JW. and Bennink, JR. 1990. Cell. 62: 203-6. PMID: 1695549
2. Beta 2-microglobulin deficient mice lack CD4-8+ cytolytic T cells.  |  Zijlstra, M., et al. 1990. Nature. 344: 742-6. PMID: 2139497
3. Evolution of class-I MHC genes and proteins: from natural selection to thymic selection.  |  Lawlor, DA., et al. 1990. Annu Rev Immunol. 8: 23-63. PMID: 2188663
4. Structure of the human class I histocompatibility antigen, HLA-A2.  |  Bjorkman, PJ., et al. Nature. 329: 506-12. PMID: 3309677
5. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites.  |  Allen, H., et al. Nature. 309: 279-81. PMID: 6201750
6. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.  |  Ozato, K. and Sachs, DH. 1981. J Immunol. 126: 317-21. PMID: 6935293
7. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex.  |  Ozato, K., et al. 1980. J Immunol. 125: 2473-7. PMID: 7191868
8. CTL respond to a mitochondrial antigen presented by H2-Db.  |  Dabhi, VM. and Lindahl, KF. 1996. Immunogenetics. 45: 65-8. PMID: 8881039
9. Brain-infiltrating cytolytic T lymphocytes specific for Theiler's virus recognize H2Db molecules complexed with a viral VP2 peptide lacking a consensus anchor residue.  |  Borson, ND., et al. 1997. J Virol. 71: 5244-50. PMID: 9188592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-H2-D^b Antibody (27-11-13) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-H2-D^b Antibody (27-11-13) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

H2-D^b Antibody (27-11-13) Specifications

Alternate Product Name: H2-Db

Product Clone Name: 27-11-13

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: H2-D^b

Epitope Species: mouse

Product Reactivity/Detection: α3 domain of H2-D^b class I MHC antigen

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, FCM

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for H2-D^b Antibody (27-11-13): FITC: Fluoroscein, PE: Phycoerythrin