H2-Db Antibody (28-14-8) recommended for detection of α3 domain of H2-Db class I MHC antigen of mouse origin by IF and FCM

H2-Db Antibody (28-14-8): sc-52541

H2-Db Antibody (28-14-8) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-H2-Db Antibody (28-14-8) is a mouse monoclonal IgG2a H2-Db antibody provided at 100 µg/ml
    • raised against C3H.SW splenocytes of mouse origin
    • recommended for detection of α3 domain of H2-Db class I MHC antigen of mouse origin by IF and FCM; may cross-react with the α3 domain of H2-Ld and with H2-Dq and H2-Lq; non cross-reactive with H2-Kd or H2-Dd
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM

Can H2-Db (28-14-8): sc-52541 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, H2-Db (28-14-8): sc-52541 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great antibody This antibody worked adequately on our blot, works very well in WB, used 1:500 dilution.
Date published: 2017-02-07
Rated 4 out of 5 by from Produced wonderful indirect FCM data for Produced wonderful indirect FCM data for mouse peripheral blood leukocytes stained with H2-D<sup>b</sup> . -SCBT QC
Date published: 2014-04-25
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