H-Ras Antibody (259): sc-35

H-Ras Antibody (259) is a rat monoclonal IgG1 that detects H-Ras in mouse, rat, and human samples through various techniques including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). H-Ras is a small GTPase that plays a crucial role in regulating signal transduction pathways related to cell growth, differentiation, and apoptosis. The subcellular localization of H-Ras is particularly important for its function. H-Ras primarily resides at the plasma membrane, where H-Ras is activated to initiate signaling cascades. H-Ras can also localize to the endoplasmic reticulum and Golgi apparatus, where H-Ras can engage different signaling pathways, affecting processes such as proliferation and transformation. The correct localization is achieved through post-translational modifications, including palmitoylation and farnesylation, which facilitate membrane attachment. Disruption of this localization, for example, by inhibiting the palmitoylation process, can impair H-Ras signaling, underscoring H-Ras dependence on membrane association for effective function. H-Ras (259) monoclonal antibody is available in both non-conjugated and multiple conjugated forms, making H-Ras (259) antibody versatile for various experimental applications, including the detection and study of H-Ras in different cellular compartments and under different experimental conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

H-Ras Antibody (259) References:

1. GTP-dependent segregation of H-ras from lipid rafts is required for biological activity.  |  Prior, IA., et al. 2001. Nat Cell Biol. 3: 368-75. PMID: 11283610
2. H-Ras signaling and K-Ras signaling are differentially dependent on endocytosis.  |  Roy, S., et al. 2002. Mol Cell Biol. 22: 5128-40. PMID: 12077341
3. Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors.  |  Arozarena, I., et al. 2004. Mol Cell Biol. 24: 1516-30. PMID: 14749369
4. Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane.  |  Rotblat, B., et al. 2004. Mol Cell Biol. 24: 6799-810. PMID: 15254246
5. Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signaling.  |  Roy, S., et al. 2005. Mol Cell Biol. 25: 6722-33. PMID: 16024806
6. H-Ras dynamically interacts with recycling endosomes in CHO-K1 cells: involvement of Rab5 and Rab11 in the trafficking of H-Ras to this pericentriolar endocytic compartment.  |  Gomez, GA. and Daniotti, JL. 2005. J Biol Chem. 280: 34997-5010. PMID: 16079139
7. A novel switch region regulates H-ras membrane orientation and signal output.  |  Abankwa, D., et al. 2008. EMBO J. 27: 727-35. PMID: 18273062
8. Nonsteroidal anti-inflammatory drugs alter the spatiotemporal organization of Ras proteins on the plasma membrane.  |  Zhou, Y., et al. 2012. J Biol Chem. 287: 16586-95. PMID: 22433858
9. H-ras distribution and signaling in plasma membrane microdomains are regulated by acylation and deacylation events.  |  Agudo-Ibáñez, L., et al. 2015. Mol Cell Biol. 35: 1898-914. PMID: 25776558
10. Individual S-acylated cysteines differentially contribute to H-Ras endomembrane trafficking and acylation/deacylation cycles.  |  Pedro, MP., et al. 2017. Mol Biol Cell. 28: 962-974. PMID: 28179458

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-H-Ras Antibody (259) has 184 citations in a variety of scientific publications.

Primary antibodies like Anti-H-Ras Antibody (259) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

H-Ras Antibody (259) Specifications

Alternate Product Name: H-Ras

Product Clone Name: 259

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 62-76

Epitope Target: H-Ras

Epitope Species: human

Product Reactivity/Detection: antigenic determinants common to H-, K- and N-Ras p21

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 184 citations in various scientific publications

Available Conjugates/Forms for H-Ras Antibody (259): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, P: Blocking Peptide, PE: Phycoerythrin

The preferred pan Ras antibody is pan Ras Antibody (F132): sc-32.