GW182 Antibody (G-10): sc-374459

GW182 Antibody (G-10) is a mouse monoclonal IgG3 antibody that detects GW182 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GW182 is a critical cytoplasmic marker protein for GW bodies (GWBs), which serve as essential storage centers and degradation sites for messenger RNAs (mRNAs). The proper functioning of GW182 is vital for the maintenance and stability of GWB structures, as any disassembly or disruption of these bodies can significantly impair the silencing activity of small interfering RNAs (siRNAs) and microRNAs (miRNAs). This is particularly important in cellular processes that regulate gene expression and maintain cellular homeostasis. GW182 is ubiquitously expressed and has been shown to interact with endogenous argonaute-2 (Ago2), a key player in the RNA-induced silencing complex, which is also enriched in GWBs. Notably, GW182 has been implicated in various autoimmune conditions, as patients with ataxia, Sjögren′s syndrome, and sensor neuropathy disease often develop autoantibodies against proteins associated with GWB structures. Anti-GW182 antibody (G-10) provides a reliable tool for researchers studying the intricate roles of GWBs and their associated proteins in gene regulation and disease mechanisms.

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GW182 Antibody (G-10) References:

1. Clinical and serological associations of autoantibodies to GW bodies and a novel cytoplasmic autoantigen GW182.  |  Eystathioy, T., et al. 2003. J Mol Med (Berl). 81: 811-8. PMID: 14598044
2. Importance of the C-terminal domain of the human GW182 protein TNRC6C for translational repression.  |  Zipprich, JT., et al. 2009. RNA. 15: 781-93. PMID: 19304925
3. The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.  |  Lian, SL., et al. 2009. RNA. 15: 804-13. PMID: 19324964
4. A C-terminal silencing domain in GW182 is essential for miRNA function.  |  Eulalio, A., et al. 2009. RNA. 15: 1067-77. PMID: 19383769
5. The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release.  |  Zekri, L., et al. 2009. Mol Cell Biol. 29: 6220-31. PMID: 19797087
6. Mapping of Ago2-GW182 functional interactions.  |  Yao, B., et al. 2011. Methods Mol Biol. 725: 45-62. PMID: 21528446
7. GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets.  |  Braun, JE., et al. 2011. Mol Cell. 44: 120-33. PMID: 21981923
8. miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.  |  Chekulaeva, M., et al. 2011. Nat Struct Mol Biol. 18: 1218-26. PMID: 21984184
9. Ethanol facilitates hepatitis C virus replication via up-regulation of GW182 and heat shock protein 90 in human hepatoma cells.  |  Bukong, TN., et al. 2013. Hepatology. 57: 70-80. PMID: 22898980
10. Acute necrotizing encephalopathy-linked mutations in Nup358 impair interaction of Nup358 with TNRC6/GW182 and miRNA function.  |  Deshmukh, P., et al. 2021. Biochem Biophys Res Commun. 559: 230-237. PMID: 33962210

The preferred GW182 antibody is GW182 Antibody (4B6): sc-56314.