Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gα o CRISPR/Cas9 KO Plasmid (h): sc-400897
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gα o CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gα o genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gα o Antibody (A2): sc-13532
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gα o CRISPR/Cas9 KO Plasmid (h) | sc-400897 | 20 µg | $397.00 |
Overview
GNAO1 encodes the human heterotrimeric G protein alpha subunit Gαo, a dominant Gi/o-family signaling component enriched in neurons that couples activated GPCRs to inhibition of adenylyl cyclase and modulation of ion channels. Through regulation of cAMP/PKA signaling, Ca2+ and K+ channel activity, and downstream MAPK and neurotransmitter release pathways, Gαo shapes synaptic transmission, neuronal excitability, and network development. Altered GNAO1 function is linked to neurodevelopmental and movement disorder phenotypes, making it a key node for interrogating GPCR-driven signaling architecture in cellular and neuronal models. Experimental disruption of GNAO1 supports mechanistic studies of receptor coupling specificity, second-messenger dynamics, and activity-dependent transcriptional responses.
Gα o CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GNAO1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GNAO1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GNAO1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gα o protein expression.
This CRISPR knockout system enables efficient generation of GNAO1-deficient cell models for investigation of Gα o signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GNAO1 exon(s) critical for Gα o function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GNAO1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gα o CRISPR/Cas9 KO Plasmid (h) and Gα o CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GNAO1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gα o HDR Plasmid (h) and Gα o HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GNAO1 homology arms to support homology-directed repair at defined GNAO1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.