Gα 15 Double Nickase Plasmid (h): sc-403266-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gα 15 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gα 15 Double Nickase Plasmid (h) and Gα 15 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GNA15. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gα 15 Antibody (F-3): sc-393878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gα 15 Double Nickase Plasmid (h) | sc-403266-NIC | 20 µg | $410.00 | |||
Gα 15 Double Nickase Plasmid (h2) | sc-403266-NIC-2 | 20 µg | $410.00 |
GNA15 encodes the heterotrimeric G protein alpha subunit Gα15, a member of the Gq family that couples activated GPCRs to phospholipase C-β, stimulating IP3/DAG production, intracellular Ca2+ mobilization, and PKC-dependent signaling. This axis influences downstream MAPK/ERK and other Ca2+-regulated transcriptional programs that shape immune cell activation, secretion, and chemotactic responses. Gα15 is enriched in hematopoietic lineages and is frequently used as a coupling surrogate to link diverse GPCRs to calcium readouts, facilitating pathway deconvolution. Dysregulated GPCR–G protein signaling involving GNA15 has been implicated across inflammatory signaling contexts and in mechanistic studies of aberrant proliferation and differentiation programs.
Gα 15 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GNA15 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GNA15. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GNA15 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GNA15-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.