GSTT1 CRISPR/Cas9 KO Plasmid (m): sc-420721
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTT1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GSTT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTT1 CRISPR/Cas9 KO Plasmid (m) | sc-420721 | 20 µg | $397.00 |
Overview
Mouse Gstt1 encodes glutathione S-transferase theta 1 (GSTT1), a phase II detoxification enzyme that catalyzes glutathione conjugation to a range of electrophilic xenobiotics and reactive endogenous metabolites. GSTT1 contributes to redox homeostasis and cellular defense against oxidative stress by facilitating clearance of lipid peroxidation products and other reactive intermediates. Its activity intersects with glutathione metabolism, chemical carcinogenesis response pathways, and broader electrophile/ROS signaling networks that influence cellular stress adaptation. Variation in GSTT family function is frequently studied in the context of toxicant sensitivity, inflammation-associated tissue injury, and tumor biology models where detoxification capacity can modify phenotypes.
GSTT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gstt1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gstt1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gstt1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GSTT1 protein expression.
This CRISPR knockout system enables efficient generation of Gstt1-deficient cell models for investigation of GSTT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gstt1 exon(s) critical for GSTT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gstt1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GSTT1 CRISPR/Cas9 KO Plasmid (m) and GSTT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gstt1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GSTT1 HDR Plasmid (m) and GSTT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gstt1 homology arms to support homology-directed repair at defined Gstt1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.