Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSTP1 Double Nickase Plasmid (h): sc-402084-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSTP1 Double Nickase Plasmid (h) and GSTP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSTP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSTP1 Antibody (3F2C2): sc-66000
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTP1 Double Nickase Plasmid (h) | sc-402084-NIC | 20 µg | $410.00 | |||
GSTP1 Double Nickase Plasmid (h2) | sc-402084-NIC-2 | 20 µg | $410.00 |
GSTP1 encodes glutathione S-transferase pi 1, a cytosolic phase II detoxification enzyme that catalyzes glutathione conjugation to electrophilic compounds, supporting redox homeostasis and cellular defense against oxidative stress. GSTP1 participates in xenobiotic metabolism and modulates stress-activated signaling, including regulation of MAPK pathways such as JNK through protein–protein interactions. Altered GSTP1 expression or promoter methylation has been associated with changes in antioxidant capacity and cellular stress responses across multiple cancer-relevant contexts, where it is often studied as a marker of detoxification status. These functions make GSTP1 a useful node for investigating metabolic stress, drug response mechanisms, and redox-regulated signaling networks in human cells.
GSTP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSTP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSTP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSTP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSTP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.