Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSTM4 Double Nickase Plasmid (h): sc-405305-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTM4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSTM4 Double Nickase Plasmid (h) and GSTM4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSTM4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSTM4 Antibody (PL-B12): sc-134353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTM4 Double Nickase Plasmid (h) | sc-405305-NIC | 20 µg | $410.00 | |||
GSTM4 Double Nickase Plasmid (h2) | sc-405305-NIC-2 | 20 µg | $410.00 |
GSTM4 encodes a Mu class glutathione S-transferase that catalyzes conjugation of reduced glutathione to electrophilic compounds, supporting phase II detoxification and redox homeostasis. By metabolizing lipid peroxidation products and xenobiotic intermediates, GSTM4 contributes to cellular defense against oxidative and chemical stress and helps regulate intracellular reactive oxygen species. Its activity interfaces with glutathione metabolism and broader antioxidant response programs that influence signaling, mitochondrial function, and cell survival. Altered GSTM4 expression or function has been linked in the literature to variability in xenobiotic handling and stress tolerance, making it relevant for mechanistic studies of toxicant response and cancer-associated metabolic remodeling.
GSTM4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSTM4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSTM4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSTM4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSTM4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.