Date published: 2025-9-7
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GST Antibody (B-14) - Western Blotting - Image 389635
GST 抗体 (B-14) Alexa Fluor® 790: sc-138 AF790. Stat4 人类重组蛋白 (A) 和日本血吸虫重组蛋白 (B) GST 融合蛋白中 GST 表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
GST 抗体 (B-14) Alexa Fluor® 790: sc-138 AF790. Stat4 人类重组蛋白 (A) 和日本血吸虫重组蛋白 (B) GST 融合蛋白中 GST 表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.

GST 抗体 (B-14): sc-138

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说明书
  • GST 抗体 B-14 是小鼠单克隆 IgG1 κ，GST抗体, 在1,414篇文献中引用，规格为200 µg/ml
  • 针对pGEX.3X重组载体编码的融合蛋白的26 kDa GST特异性结构域的抗体
  • GST 抗体 (B-14) 推荐用于WB 和 IP，检测Schistosoma japonicum 来源的 GST fusion proteins and glutathione-S-transferase (GST); 专门设计用于GST表达载体，如pGEX.3X和pGEX.2T
  • 抗GST抗体（B-14）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 和 ELISA；偶联TRITC 用于IF, IHC(P) 和 FCM
  • 关于如何获取GST (B-14): sc-138的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是GST Antibody (B-14) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与GST Antibody (B-14) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

谷胱甘肽转移酶抗体（B-14）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测日本血吸虫的谷胱甘肽转移酶（GST）。抗谷胱甘肽转移酶抗体（B-14）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。谷胱甘肽转移酶（GST）通过催化谷胱甘肽与各种底物的结合，在有害化合物解毒中发挥着至关重要的作用，这对于细胞抵抗氧化应激和外来生物损伤至关重要。这种功能在日本血吸虫中尤为重要，因为谷胱甘肽转移酶（GST）通过中和潜在的有害物质，帮助寄生虫在宿主环境中存活。此外，质粒载体（如pGEX.3X）通常用于表达包括GST在内的混合融合蛋白，从而促进对蛋白质相互作用和功能的研究。GST (B-14)抗体对于验证斑块和蛋白质印迹（WB）中的蛋白质表达以及大肠杆菌中表达的蛋白质的免疫亲和纯化都是非常宝贵的，这使得GST (B-14)单克隆抗体成为该领域研究人员必不可少的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GST 抗体 (B-14) 参考文献:

  1. 用 Pichia pastoris 表达融合了 GST 的人 Csk 同源激酶的激酶结构域便于纯化。  |  Murthy, TV. 2004. Biotechnol Lett. 26: 443-9. PMID: 15104145
  2. 肾细胞癌中谷胱甘肽-S-转移酶-pi（GST-pi）的表达。  |  Kaprilian, C., et al. 2015. J Kidney Cancer VHL. 2: 25-29. PMID: 28326256
  3. 单步纯化在大肠杆菌中表达的与谷胱甘肽 S-转移酶融合的多肽。  |  Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
  4. 谷胱甘肽-S-转移酶（GST）催化吉林克雷伯氏菌 2N3 降解乙嘧磺隆。  |  Zhang, S., et al. 2020. Sci Total Environ. 729: 139075. PMID: 32388135
  5. 使用谷胱甘肽-S-转移酶（GST）下拉检测技术检测蛋白质-蛋白质相互作用。  |  Gnanasekaran, P. and Pappu, HR. 2023. Methods Mol Biol. 2690: 111-115. PMID: 37450141
  6. 谷胱甘肽S-转移酶（GST）基因的多态性可改变孕期接触母体吸烟代谢物对母体和后代DNA甲基化的影响。  |  Kheirkhah Rahimabad, P., et al. 2023. Genes (Basel). 14: PMID: 37628696
  7. 线粒体泛醌-细胞色素 c 还原酶小分子质量泛醌结合蛋白的克隆, 基因测序和表达。  |  Yu, L., et al. 1995. J Biol Chem. 270: 25634-8. PMID: 7592738
  8. 在大肠杆菌中表达马疱疹病毒 1 糖蛋白 C 的小区域。  |  Crabb, BS. and Studdert, MJ. 1995. Vet Microbiol. 46: 181-91. PMID: 8545955
  9. 评估小鼠巨细胞病毒糖蛋白 B 的抗原性和遗传变异。  |  Xu, J., et al. 1996. J Gen Virol. 77 (Pt 1): 49-59. PMID: 8558128
  10. 母细胞蛋白：表达, 纯化和特征描述。  |  Soler, D., et al. 1995. J Protein Chem. 14: 511-20. PMID: 8561847
  11. sphaeroides 罗杆菌细胞色素 b-c1 复合物亚基 IV 的功能表达及重组蛋白与三亚基核心复合物的重组。  |  Chen, YR., et al. 1996. J Biol Chem. 271: 2057-62. PMID: 8567659
  12. 原生和重组人唾液组蛋白 1 的功能比较。  |  Driscoll, J., et al. 1995. J Dent Res. 74: 1837-44. PMID: 8600179

订购信息

产品名称产品编号规格价格数量收藏夹

GST 抗体 (B-14)

sc-138
200 µg/ml
$316.00

GST (B-14): m-IgG Fc BP-HRP 套装

sc-528100
200 µg Ab; 10 µg BP
$354.00

GST (B-14): m-IgGκ BP-HRP 套装

sc-520398
200 µg Ab, 40 µg BP
$354.00

GST (B-14): m-IgG1 BP-HRP 套装

sc-542758
200 µg Ab; 20 µg BP
$354.00

GST 抗体 (B-14) AC

sc-138 AC
500 µg/ml, 25% agarose
$416.00

GST 抗体 (B-14) HRP

sc-138 HRP
200 µg/ml
$316.00

GST 抗体 (B-14) FITC

sc-138 FITC
200 µg/ml
$330.00

GST 抗体 (B-14) PE

sc-138 PE
200 µg/ml
$343.00

GST 抗体 (B-14) Alexa Fluor® 488

sc-138 AF488
200 µg/ml
$357.00

GST 抗体 (B-14) Alexa Fluor® 546

sc-138 AF546
200 µg/ml
$357.00

GST 抗体 (B-14) Alexa Fluor® 594

sc-138 AF594
200 µg/ml
$357.00

GST 抗体 (B-14) Alexa Fluor® 647

sc-138 AF647
200 µg/ml
$357.00

GST 抗体 (B-14) Alexa Fluor® 680

sc-138 AF680
200 µg/ml
$357.00

GST 抗体 (B-14) Alexa Fluor® 790

sc-138 AF790
200 µg/ml
$357.00

GST 抗体 (B-14) B

sc-138 B
200 µg/ml
$320.00

GST 抗体 (B-14) TRITC

sc-138 TRITC
200 µg/ml
$320.00

May I know if this GST antibody can be used to probe GST-tagged proteins expressed in drosophila S2 cells system? Thank you

Asked by: keke
Thank you for your question. sc-138 GST (B-14) has not been validated for drosophila.
Answered by: BlakeJ
Date published: 2024-08-23

こちらはアジ化ナトリウム0.1%未満含有とのことですが、毒物及び劇物指定令第一条の１にある「アジ化ナトリウム及びこれを含有する製剤。ただし、アジ化ナトリウム0.1%以下を含有するものを除く。」に該当しますか？

Asked by: KT0828
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I want to stain splenocytes with recombinant GST-fused receptor X to cell-sort bound population with flow cytometry. To aim this, I need to buy anti-GST antibody which recognizes native GST structure. Is this clone good for this purpose?

Asked by: Oklahoma City
Thank you for your question. We do not know if this monoclonal antibody will recognize native GST structure.
Answered by: Technical Support
Date published: 2019-09-11

I purchased AF488-labeled anti-GST antibody (B-14) for detecting the binding of my GST-fused protein on cells expressing ligand by flow cytometry (FCM). But I did not see AF488 staining. Does this B-14 work ok to recognize native GST structure?

Asked by: yutanOK
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-04-20

Can it be used for IF in cell lines? If not, do you have GST antibody for IF?

Asked by: Noddang
Thank you for your question. This antibody is not recommended for IF. We recommend sc-374171 as a good alternative for this application.
Answered by: Tech Service
Date published: 2019-02-25

I would like to know which is the epitope that it is recognized by the antibody. I have to include this epitope as a control in a peptide array. Thanks.

Asked by: Emiliano
Thank you for your question. This antibody was raised against the 26 kDa GST specific domain of a fusion protein encoded by a pGEX.3X recombinant vector. The exact epitope has not been epitope mapped. If you have any further questions, please contact Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Will the antibody GST Antibody (B-14): sc-138 work against the antioxidant enzyme, glutathione S transferase in Western blot studies?

Asked by: Nilanjana
Thank you for your question. Yes, this mouse monoclonal antibody, GST (B-14): sc-138, is recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by Western Blot (WB) and immunoprecipitation (IP).
Answered by: Technical Support
Date published: 2017-11-17

endogenous gst도 detection 이 되나요

Asked by: aoan
문의 주셔서 감사합니다. Schistosoma japonicum의 endogenous GST를 detection할수 있습니다. 감사합니다.
Answered by: Technical Support 13
Date published: 2017-08-04
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Rated 5 out of 5 by from GST Antibody (B-14): sc-138Very strong signal obtained for detection of tagged recombinant proteins from human cells. The signal was totally absent in the negative controls. Works very well even with very high dilution. Blocking of membrane with 5% milk suits the antibody very well.
Date published: 2023-02-08
Rated 5 out of 5 by from Anti-GST AntibodyStrong signal for purified proteins on Western Blotting!
Date published: 2022-01-06
Rated 4 out of 5 by from Works greatWork well in western blotting to detect tagged recombinant proteins
Date published: 2021-07-24
Rated 5 out of 5 by from きちんとworkする抗体ウェスタンでこれはきちんとworkします。他者の抗体も試したけど、GSTに対する抗体だし、コスパと綺麗さを考えたらこれがいい。
Date published: 2021-06-15
Rated 5 out of 5 by from Worked perfect in WBAs expected worked perfectly in Westernblot with GST recombinant proteins purified from bacteria.
Date published: 2021-03-10
Rated 5 out of 5 by from Works really well on western blotI used this on western blot to visualize GST-tagged proteins from bacteria after IPTG induction. It work very well at 1:2000 dilution. I guess can be used even more diluted.
Date published: 2021-03-05
Rated 5 out of 5 by from Works great for WBWe routinely use the antibody in WB to detect GST-tagged S. cerevisiae proteins expressed in E. coli; works well at 1:2000
Date published: 2020-01-22
Rated 5 out of 5 by from Great for WBWe routinely use the antibody in WB to detect GST-tagged S. cerevisiae proteins expressed in E. coli; works well at 1:2000
Date published: 2020-01-09
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GST 抗体 (B-14) is rated 4.8 out of 5 by 47.
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