Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSS Double Nickase Plasmid (h): sc-402992-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSS Double Nickase Plasmid (h) and GSS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSS Antibody (H-7): sc-166882
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSS Double Nickase Plasmid (h) | sc-402992-NIC | 20 µg | $410.00 | |||
GSS Double Nickase Plasmid (h2) | sc-402992-NIC-2 | 20 µg | $410.00 |
Glutathione synthetase (GSS) encodes the ATP-dependent enzyme that catalyzes the final step of glutathione biosynthesis, ligating glycine to γ-glutamylcysteine to produce reduced glutathione (GSH). By controlling intracellular GSH pools, GSS supports cellular redox homeostasis, detoxification of electrophiles, and maintenance of thiol-dependent signaling under oxidative stress. GSS activity intersects with glutathione metabolism and broader antioxidant defense networks that influence mitochondrial function, protein S-glutathionylation, and responses to xenobiotics. Altered GSS function has been associated with inborn errors of glutathione biosynthesis and phenotypes linked to heightened oxidative damage, making it relevant for mechanistic studies of stress sensitivity and metabolic vulnerability.
GSS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.