GSDMDC1 Double Nickase Plasmid (h): sc-406596-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSDMDC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSDMDC1 Double Nickase Plasmid (h) and GSDMDC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSDMD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSDMDC1 Antibody (H-11): sc-393581
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSDMDC1 Double Nickase Plasmid (h) | sc-406596-NIC | 20 µg | $410.00 | |||
GSDMDC1 Double Nickase Plasmid (h2) | sc-406596-NIC-2 | 20 µg | $410.00 |
GSDMD encodes gasdermin D, a pore-forming effector that is proteolytically activated downstream of inflammasome signaling and caspase-1/4/5/11 cleavage to execute pyroptotic cell death. Upon activation, the N-terminal fragment oligomerizes in the plasma membrane to form pores that drive ionic flux, cell swelling, and release of pro-inflammatory mediators such as IL-1 family cytokines, linking innate immune sensing to inflammatory outcomes. This pathway intersects with pattern-recognition receptor signaling, NF-κB priming, and noncanonical inflammasome activation, shaping antimicrobial defense and sterile inflammation. Dysregulated gasdermin D activity is implicated in inflammatory and infectious disease biology and is widely studied in myeloid cells, epithelial barriers, and tumor-immune microenvironments.
GSDMDC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSDMD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSDMD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSDMD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSDMD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.