GRP 94 Antibody (9G10): sc-32249

GRP 94 Antibody (9G10) is a rat monoclonal IgG2a antibody that detects GRP 94 protein of mouse, rat, human, and chicken origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-GRP 94 antibody (9G10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRP 94 protein, also known as heat shock protein 90kDa beta member 1 (HSP90B1), plays a crucial role as a molecular chaperone located in the endoplasmic reticulum (ER), where GRP 94 assists in proper folding and maturation of proteins, particularly those involved in cell signaling and immune responses. GRP 94′s localization in the ER ensures proteins are correctly processed before transport to final destinations, which is essential for maintaining cellular homeostasis and responding to stress conditions. GRP 94 is particularly important in cancer, as GRP 94 is often overexpressed in tumor cells, aiding in survival and proliferation under adverse conditions. GRP 94 interacts with various proteins, including tumor rejection antigen 1 (TRA1) and TNF receptor-associated protein 1 (TRAP1), further highlighting GRP 94′s significance in cellular processes and potential as a therapeutic target.

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GRP 94 Antibody (9G10) References:

1. PKC epsilon is a unique regulator for hsp90 beta gene in heat shock response.  |  Wu, JM., et al. 2003. J Biol Chem. 278: 51143-9. PMID: 14532285
2. HSP90 and the chaperoning of cancer.  |  Whitesell, L. and Lindquist, SL. 2005. Nat Rev Cancer. 5: 761-72. PMID: 16175177
3. Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi.  |  Cowen, LE. and Lindquist, S. 2005. Science. 309: 2185-9. PMID: 16195452
4. Modulating molecular chaperone Hsp90 functions through reversible acetylation.  |  Aoyagi, S. and Archer, TK. 2005. Trends Cell Biol. 15: 565-7. PMID: 16199163
5. The HSP90 family of genes in the human genome: insights into their divergence and evolution.  |  Chen, B., et al. 2005. Genomics. 86: 627-37. PMID: 16269234
6. Hsp90: a chaperone for protein folding and gene regulation.  |  Zhao, R. and Houry, WA. 2005. Biochem Cell Biol. 83: 703-10. PMID: 16333321
7. Substrate transfer from the chaperone Hsp70 to Hsp90.  |  Wegele, H., et al. 2006. J Mol Biol. 356: 802-11. PMID: 16403523
8. Modulation of chaperone function and cochaperone interaction by novobiocin in the C-terminal domain of Hsp90: evidence that coumarin antibiotics disrupt Hsp90 dimerization.  |  Allan, RK., et al. 2006. J Biol Chem. 281: 7161-71. PMID: 16421106
9. Translocation of ATP into the lumen of rough endoplasmic reticulum-derived vesicles and its binding to luminal proteins including BiP (GRP 78) and GRP 94.  |  Clairmont, CA., et al. 1992. J Biol Chem. 267: 3983-90. PMID: 1740446
10. Molecular Chaperone GRP94/GP96 in Cancers: Oncogenesis and Therapeutic Target.  |  Duan, X., et al. 2021. Front Oncol. 11: 629846. PMID: 33898309
11. FBXL2 counteracts Grp94 to destabilize EGFR and inhibit EGFR-driven NSCLC growth.  |  Niu, M., et al. 2021. Nat Commun. 12: 5919. PMID: 34635651
12. Chaperone function of a Grp 94-related protein for folding and transport of the pancreatic bile salt-dependent lipase.  |  Bruneau, N. and Lombardo, D. 1995. J Biol Chem. 270: 13524-33. PMID: 7768954

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GRP 94 Antibody (9G10) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-GRP 94 Antibody (9G10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GRP 94 Antibody (9G10) Specifications

Alternate Product Name: Glucose-regulated protein 94 kD (GRP94)

Product Clone Name: 9G10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 785-803

Epitope Target: GRP 94

Epitope Species: chicken

Product Reactivity/Detection: GRP 94

Species Reactivity/Detection: mouse, rat, human, chicken

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for GRP 94 Antibody (9G10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin