group X sPLA2 Antibody (E-4): sc-514324
- group X sPLA2 Antibody (E-4) is a mouse monoclonal IgG1 κ group X sPLA2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 81-155 mapping at the C-terminus of group X sPLA2 of human origin
- group X sPLA2 Antibody (E-4) is recommended for detection of group X sPLA2 of mouse, rat, human origin by WB, IP, IF and ELISA
- Anti-group X sPLA2 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for group X sPLA2 Antibody (E-4) for WB applications. These reagents are now offered in bundles with group X sPLA2 Antibody (E-4) (see ordering information below).
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Group X sPLA2 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects group X sPLA2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Group X sPLA2 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Group X sPLA2 plays a crucial role in regulating phospholipid metabolism and eicosanoid biosynthesis, which are essential processes in cell signaling and inflammation. Group X sPLA2 is actively secreted and encoded by a gene located on human chromosome 16p13.1-p12, with mRNA expression detected in various tissues such as lung, thymus, and spleen. Immunohistochemical analysis has shown that group X sPLA2 is expressed in splenic macrophages, highlighting group X sPLA2′s importance in immune responses. Group X sPLA2′s ability to catalyze fatty acid release from phospholipids underscores group X sPLA2′s significance in maintaining cellular membrane integrity and function, making group X sPLA2 a valuable target for research in both basic and applied sciences.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
group X sPLA2 Antibody (E-4) References:
- Human pancreatic islets express mRNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on chromosome 22q13.1. | Ma, Z., et al. 1999. J Biol Chem. 274: 9607-16. PMID: 10092647
- Mouse group X secretory phospholipase A2 induces a potent release of arachidonic acid from spleen cells and acts as a ligand for the phospholipase A2 receptor. | Morioka, Y., et al. 2000. Arch Biochem Biophys. 381: 31-42. PMID: 11019817
- Structures of free and inhibited human secretory phospholipase A2 from inflammatory exudate. | Scott, DL., et al. 1991. Science. 254: 1007-10. PMID: 1948070
- Cloning, chromosomal mapping, and expression of a novel human secretory phospholipase A2. | Cupillard, L., et al. 1997. J Biol Chem. 272: 15745-52. PMID: 9188469
- Crystal structure of human secretory phospholipase A2-IIA complex with the potent indolizine inhibitor 120-1032. | Kitadokoro, K., et al. 1998. J Biochem. 123: 619-23. PMID: 9538252
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
group X sPLA2 Antibody (E-4) | sc-514324 | 200 µg/ml | $316.00 | |||
group X sPLA2 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-527886 | 200 µg Ab; 10 µg BP | $354.00 | |||
group X sPLA2 Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-533259 | 200 µg Ab; 20 µg BP | $354.00 | |||
group X sPLA2 Antibody (E-4) AC | sc-514324 AC | 500 µg/ml, 25% agarose | $416.00 | |||
group X sPLA2 Antibody (E-4) HRP | sc-514324 HRP | 200 µg/ml | $316.00 | |||
group X sPLA2 Antibody (E-4) FITC | sc-514324 FITC | 200 µg/ml | $330.00 | |||
group X sPLA2 Antibody (E-4) PE | sc-514324 PE | 200 µg/ml | $343.00 | |||
group X sPLA2 Antibody (E-4) Alexa Fluor® 488 | sc-514324 AF488 | 200 µg/ml | $357.00 | |||
group X sPLA2 Antibody (E-4) Alexa Fluor® 546 | sc-514324 AF546 | 200 µg/ml | $357.00 | |||
group X sPLA2 Antibody (E-4) Alexa Fluor® 594 | sc-514324 AF594 | 200 µg/ml | $357.00 | |||
group X sPLA2 Antibody (E-4) Alexa Fluor® 647 | sc-514324 AF647 | 200 µg/ml | $357.00 | |||
group X sPLA2 Antibody (E-4) Alexa Fluor® 680 | sc-514324 AF680 | 200 µg/ml | $357.00 | |||
group X sPLA2 Antibody (E-4) Alexa Fluor® 790 | sc-514324 AF790 | 200 µg/ml | $357.00 |