Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
group VI iPLA2 CRISPR/Cas9 KO Plasmid (m): sc-424697
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- group VI iPLA2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the group VI iPLA2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: group VI iPLA2 Antibody (D-4): sc-376563
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
group VI iPLA2 CRISPR/Cas9 KO Plasmid (m) | sc-424697 | 20 µg | $397.00 |
Overview
Pla2g6 encodes group VI iPLA2, a Ca2+-independent phospholipase A2 that hydrolyzes membrane phospholipids to release lysophospholipids and free fatty acids, including arachidonic acid. Through lipid remodeling and signaling, it influences membrane homeostasis, mitochondrial integrity, vesicle trafficking, and downstream eicosanoid and oxidative stress pathways. In mouse systems, Pla2g6 activity has been linked to neurodegenerative phenotypes, inflammation-associated lipid mediator production, and susceptibility to membrane damage in metabolically active tissues. These functions make Pla2g6 a useful node for studying lipid-driven regulation of cell stress responses and neuroinflammation-relevant processes.
group VI iPLA2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pla2g6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pla2g6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pla2g6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish group VI iPLA2 protein expression.
This CRISPR knockout system enables efficient generation of Pla2g6-deficient cell models for investigation of group VI iPLA2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pla2g6 exon(s) critical for group VI iPLA2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pla2g6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by group VI iPLA2 CRISPR/Cas9 KO Plasmid (m) and group VI iPLA2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pla2g6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by group VI iPLA2 HDR Plasmid (m) and group VI iPLA2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pla2g6 homology arms to support homology-directed repair at defined Pla2g6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.