GRIP-1 CRISPR/Cas9 KO Plasmid (m): sc-421837

Overview

Ncoa2 encodes GRIP-1 (glucocorticoid receptor–interacting protein 1), a nuclear receptor coactivator that coordinates hormone-dependent transcription by bridging ligand-bound receptors to chromatin-modifying complexes and the basal transcriptional machinery. In mouse cells, GRIP-1 functions within steroid hormone signaling networks, including glucocorticoid, estrogen, androgen, thyroid hormone, and retinoic acid receptor pathways, influencing gene programs that regulate development, metabolism, immune responses, and cellular differentiation. As a member of the SRC/p160 coactivator family, GRIP-1 integrates signals through interactions with histone acetyltransferases and mediator-associated factors to modulate enhancer and promoter activity. Dysregulated nuclear receptor coactivation and altered transcriptional control involving NCOA2-family proteins have been linked to aberrant growth and lineage programs, making Ncoa2 a relevant target for mechanistic studies of transcriptional regulation in disease-relevant models.

GRIP-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ncoa2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ncoa2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ncoa2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRIP-1 protein expression.

This CRISPR knockout system enables efficient generation of Ncoa2-deficient cell models for investigation of GRIP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Ncoa2 exon(s) critical for GRIP-1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Ncoa2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by GRIP-1 CRISPR/Cas9 KO Plasmid (m) and GRIP-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ncoa2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by GRIP-1 HDR Plasmid (m) and GRIP-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ncoa2 homology arms to support homology-directed repair at defined Ncoa2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.