GRIN1 CRISPR/Cas9 KO Plasmid (h): sc-405452
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRIN1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GRIN1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRIN1 CRISPR/Cas9 KO Plasmid (h) | sc-405452 | 20 µg | $397.00 |
Overview
GPRIN1 (G protein regulated inducer of neurite outgrowth 1) encodes a neuron-enriched cytoplasmic protein that associates with membrane signaling complexes to couple activated G proteins to cytoskeletal remodeling. It is implicated in neurite extension, growth cone dynamics, and synaptic plasticity through regulation of actin-dependent processes and receptor-proximal signaling events. GPRIN1 has been linked to neuronal development and excitability-related pathways and is studied in the context of neurodevelopmental phenotypes and neurodegenerative mechanisms where altered neurite architecture and synaptic function contribute to disease biology. These properties make GPRIN1 a useful target for dissecting signaling-to-structure coupling in human neuronal models.
GRIN1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPRIN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPRIN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPRIN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRIN1 protein expression.
This CRISPR knockout system enables efficient generation of GPRIN1-deficient cell models for investigation of GRIN1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPRIN1 exon(s) critical for GRIN1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPRIN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GRIN1 CRISPR/Cas9 KO Plasmid (h) and GRIN1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPRIN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GRIN1 HDR Plasmid (h) and GRIN1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPRIN1 homology arms to support homology-directed repair at defined GPRIN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.