Grancalcin Antibody (G-1): sc-365835

Grancalcin Antibody (G-1) is a mouse monoclonal IgG2b that detects Grancalcin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Grancalcin is a calcium-binding protein predominantly found in human neutrophils and plays a crucial role in the immune response. Grancalcin belongs to the penta EF-hand subfamily of EF-hand proteins, which includes calpain, sorcin, peflin, and ALG-2. Notably, Grancalcin undergoes significant conformational changes upon calcium binding, which exposes hydrophobic amino acid residues that facilitate interaction with hydrophobic surfaces, thereby influencing cellular processes. Grancalcin forms a homodimer through the association of unpaired EF5 hands, enhancing functional capacity. Grancalcin interacts with L-plastin, a protein known for actin bundling activity, suggesting a role in the regulation of neutrophil adhesion and migration during immune responses. Expression is primarily associated with cells derived from the bone marrow, particularly neutrophils and monocytes, with lower levels found in lymphocytes, highlighting a specialized role in innate immunity. Anti-Grancalcin antibody (G-1) is an essential tool for researchers studying the dynamics of neutrophil function and the broader implications of calcium signaling in immune responses.

The preferred Grancalcin antibody is Grancalcin Antibody (G-3): sc-365808.