GRAMD4 Antibody (C-8): sc-515128
- GRAMD4 Antibody (C-8) is a mouse monoclonal IgG1 κ GRAMD4 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 470-570 mapping near the C-terminus of GRAMD4 of human origin
- GRAMD4 Antibody (C-8) is recommended for detection of GRAMD4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GRAMD4 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GRAMD4 Antibody (C-8) for WB applications. These reagents are now offered in bundles with GRAMD4 Antibody (C-8) (see ordering information below).
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GRAMD4 Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects GRAMD4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRAMD4 Antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The gene encoding GRAMD4, known as GRAM domain-containing protein 4, is located on human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. GRAMD4, also referred to as death-inducing protein (DIP), is a 578 amino acid protein situated in the mitochondrial membrane, playing a crucial role in mediating the p53-independent E2F-1 death pathway, a mechanism often disrupted in various cancers. GRAMD4 triggers a robust apoptotic response through the activation of caspase-3 and the cleavage of poly(ADP-ribose) polymerase, highlighting potential as a therapeutic target in cancer treatment. GRAMD4 is expressed in lung tissue and is up-regulated in primary lung squamous cell carcinoma following E2F-1 activation, particularly after the administration of 4-hydroxytamoxifen. GRAMD4 has two isoforms generated through alternative splicing, which may contribute to functional diversity and regulatory mechanisms in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GRAMD4 Antibody (C-8) References:
- Chromosome 22 workshop report. | Schwab, SG. and Wildenauer, DB. 1999. Am J Med Genet. 88: 276-8. PMID: 10374745
- Disease genes and chromosomes: disease maps of the human genome. Chromosome 22. | Gilbert, F. 1998. Genet Test. 2: 89-97. PMID: 10464604
- Constitutional rearrangements of chromosome 22 as a cause of neurofibromatosis 2. | Tsilchorozidou, T., et al. 2004. J Med Genet. 41: 529-34. PMID: 15235024
- A novel mitochondrial protein DIP mediates E2F1-induced apoptosis independently of p53. | Stanelle, J., et al. 2005. Cell Death Differ. 12: 347-57. PMID: 15565177
- Tbx1 haploinsufficiency is linked to behavioral disorders in mice and humans: implications for 22q11 deletion syndrome. | Paylor, R., et al. 2006. Proc Natl Acad Sci U S A. 103: 7729-34. PMID: 16684884
- Analyses of the associations between the genes of 22q11 deletion syndrome and schizophrenia. | Arinami, T. 2006. J Hum Genet. 51: 1037-1045. PMID: 16969581
- Mutational spectrum of the NF2 gene: a meta-analysis of 12 years of research and diagnostic laboratory findings. | Ahronowitz, I., et al. 2007. Hum Mutat. 28: 1-12. PMID: 16983642
- BCR and its mutants, the reciprocal t(9;22)-associated ABL/BCR fusion proteins, differentially regulate the cytoskeleton and cell motility. | Zheng, X., et al. 2006. BMC Cancer. 6: 262. PMID: 17090304
- Deletion 22q11: spectrum of associated disorders. | Hay, BN. 2007. Semin Pediatr Neurol. 14: 136-9. PMID: 17980310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRAMD4 Antibody (C-8) | sc-515128 | 200 µg/ml | $322.00 | |||
GRAMD4 Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-527987 | 200 µg Ab; 10 µg BP | $361.00 | |||
GRAMD4 Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-533360 | 200 µg Ab; 20 µg BP | $361.00 | |||
GRAMD4 Antibody (C-8) AC | sc-515128 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GRAMD4 Antibody (C-8) HRP | sc-515128 HRP | 200 µg/ml | $322.00 | |||
GRAMD4 Antibody (C-8) FITC | sc-515128 FITC | 200 µg/ml | $336.00 | |||
GRAMD4 Antibody (C-8) PE | sc-515128 PE | 200 µg/ml | $349.00 | |||
GRAMD4 Antibody (C-8) Alexa Fluor® 488 | sc-515128 AF488 | 200 µg/ml | $364.00 | |||
GRAMD4 Antibody (C-8) Alexa Fluor® 546 | sc-515128 AF546 | 200 µg/ml | $364.00 | |||
GRAMD4 Antibody (C-8) Alexa Fluor® 594 | sc-515128 AF594 | 200 µg/ml | $364.00 | |||
GRAMD4 Antibody (C-8) Alexa Fluor® 647 | sc-515128 AF647 | 200 µg/ml | $364.00 | |||
GRAMD4 Antibody (C-8) Alexa Fluor® 680 | sc-515128 AF680 | 200 µg/ml | $364.00 | |||
GRAMD4 Antibody (C-8) Alexa Fluor® 790 | sc-515128 AF790 | 200 µg/ml | $364.00 |