GRAMD1C CRISPR/Cas9 KO Plasmid (h): sc-412329
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRAMD1C CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GRAMD1C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRAMD1C CRISPR/Cas9 KO Plasmid (h) | sc-412329 | 20 µg | $397.00 |
Overview
GRAMD1C (GRAM domain containing 1C) encodes an endoplasmic reticulum–anchored lipid transfer protein implicated in nonvesicular sterol transport and intracellular cholesterol homeostasis. Through its GRAM domain–dependent membrane sensing and interaction with lipid-rich membranes, GRAMD1C is linked to ER–organelle contact site biology and regulation of membrane lipid composition. These processes intersect with pathways governing mitochondrial function, autophagy/mitophagy, and cellular energy metabolism, making GRAMD1C a useful node for studying lipid-driven organelle crosstalk. Altered GRAMD1C expression or function has been explored in the context of metabolic phenotypes and cancer-associated cellular states, supporting its relevance for mechanistic studies of lipid metabolism in disease models.
GRAMD1C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GRAMD1C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GRAMD1C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GRAMD1C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRAMD1C protein expression.
This CRISPR knockout system enables efficient generation of GRAMD1C-deficient cell models for investigation of GRAMD1C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GRAMD1C exon(s) critical for GRAMD1C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GRAMD1C genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GRAMD1C CRISPR/Cas9 KO Plasmid (h) and GRAMD1C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GRAMD1C locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GRAMD1C HDR Plasmid (h) and GRAMD1C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GRAMD1C homology arms to support homology-directed repair at defined GRAMD1C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.