GRAF CRISPR/Cas9 KO Plasmid (h): sc-403652
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRAF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GRAF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRAF CRISPR/Cas9 KO Plasmid (h) | sc-403652 | 20 µg | $397.00 |
Overview
ARHGAP26 encodes GRAF, a Rho GTPase-activating protein that downregulates Rho family signaling to coordinate actin cytoskeleton remodeling, membrane trafficking, and cell shape changes. Through its GAP activity and interactions with endocytic and cytoskeletal regulators, GRAF links Rho-dependent pathways to clathrin-mediated endocytosis and adhesion dynamics, influencing cell migration and polarity. Altered ARHGAP26 function has been associated with dysregulated cytoskeletal control in cancer-related processes and is also implicated in hematologic malignancies through recurrent gene rearrangements, supporting investigation of its role in signaling rewiring and cellular transformation.
GRAF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ARHGAP26 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ARHGAP26 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ARHGAP26 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRAF protein expression.
This CRISPR knockout system enables efficient generation of ARHGAP26-deficient cell models for investigation of GRAF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ARHGAP26 exon(s) critical for GRAF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ARHGAP26 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GRAF CRISPR/Cas9 KO Plasmid (h) and GRAF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ARHGAP26 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GRAF HDR Plasmid (h) and GRAF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ARHGAP26 homology arms to support homology-directed repair at defined ARHGAP26 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.