Glutathione Peroxidase 5/GPX5 Antibody (G-1): sc-390093
- Glutathione Peroxidase 5/GPX5 Antibody (G-1) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 22-66 mapping near the N-terminus of GPx-5 of human origin
- recommended for detection of GPx-5 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2a BP-HRP is the preferred secondary detection reagent for Glutathione Peroxidase 5/GPX5 Antibody (G-1) for WB applications. This reagent is now offered in a bundle with Glutathione Peroxidase 5/GPX5 Antibody (G-1) (see ordering information below).
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GPx-5 Antibody (G-1) is a mouse monoclonal IgG2a antibody that detects glutathione peroxidase 5 (GPx-5) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GPx-5 is a unique selenium-independent isoform of the glutathione peroxidase family, primarily localized in the acrosome of sperm cells, where GPx-5 plays a crucial role in protecting sperm from oxidative damage during maturation in the epididymis. This localization helps maintain sperm viability and function, which are essential for successful fertilization. GPx-5 presence in the acrosome suggests involvement in preventing premature acrosome reactions, ensuring sperm are fully prepared for fertilization. With an ability to mitigate oxidative stress, GPx-5 is vital for male fertility, making anti-GPx-5 antibody (G-1) an important tool for researchers studying reproductive biology and related disorders.
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Glutathione Peroxidase 5/GPX5 Antibody (G-1) References:
- GPX5 is present in the mouse caput and cauda epididymidis lumen at three different locations. | Rejraji, H., et al. 2002. Mol Reprod Dev. 63: 96-103. PMID: 12211066
- Expression of E-SOD, GPX5 mRNAs and immunoexpression of Cu/ZnSOD in epididymal epithelial cells of finasteride-treated rats. | Kolasa, A., et al. 2008. Andrologia. 40: 303-11. PMID: 18811921
- Nm23-M5 mediates round and elongated spermatid survival by regulating GPX-5 levels. | Choi, YJ., et al. 2009. FEBS Lett. 583: 1292-8. PMID: 19303412
- Hydroperoxide and peroxynitrite reductase activity of poplar thioredoxin-dependent glutathione peroxidase 5: kinetics, catalytic mechanism and oxidative inactivation. | Selles, B., et al. 2012. Biochem J. 442: 369-80. PMID: 22122405
- Epididymis response partly compensates for spermatozoa oxidative defects in snGPx4 and GPx5 double mutant mice. | Noblanc, A., et al. 2012. PLoS One. 7: e38565. PMID: 22719900
- Re: epididymal specific, selenium-independent GPX5 protects cells from oxidative stress-induced lipid peroxidation and DNA mutation. | Niederberger, C. 2014. J Urol. 191: 1355. PMID: 24745523
- Role of Zinc Supplementation in Testicular and Epididymal Damages in Diabetic Rat: Involvement of Nrf2, SOD1, and GPX5. | Maremanda, KP., et al. 2016. Biol Trace Elem Res. 173: 452-64. PMID: 27025721
- Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice. | Chu, C., et al. 2020. Asian J Androl. 22: 590-601. PMID: 32270769
- GPX5-Enriched Exosomes Improve Sperm Quality and Fertilization Ability. | Huang, J., et al. 2024. Int J Mol Sci. 25: PMID: 39408895
- Characterization of an androgen response element within the promoter of the epididymis-specific murine glutathione peroxidase 5 gene. | Lareyre, JJ., et al. 1997. Mol Cell Endocrinol. 129: 33-46. PMID: 9175627
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glutathione Peroxidase 5/GPX5 Antibody (G-1) | sc-390093 | 200 µg/ml | $316.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (G-1): m-IgG2a BP-HRP Bundle | sc-546068 | 200 µg Ab; 10 µg BP | $354.00 |