GPS2 CRISPR/Cas9 KO Plasmid (h): sc-406290
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPS2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPS2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPS2 CRISPR/Cas9 KO Plasmid (h) | sc-406290 | 20 µg | $397.00 |
Overview
G protein pathway suppressor 2 (GPS2) is a multifunctional adaptor protein that regulates signal-dependent transcription and integrates stress, inflammatory, and metabolic cues. It is best known as a component of the nuclear receptor corepressor (NCoR)/SMRT–HDAC3 complex, where it modulates chromatin state and represses gene expression programs downstream of pathways such as TNF/NF-κB and MAPK signaling. GPS2 also contributes to control of mitochondrial retrograde signaling and proteostasis by coordinating ubiquitin-dependent processes and transcriptional responses to cellular stress. Dysregulated GPS2 activity has been linked to altered inflammatory gene expression and metabolic phenotypes, supporting its use as a node for studying transcriptional coregulation in disease-relevant contexts.
GPS2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPS2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPS2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPS2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPS2 protein expression.
This CRISPR knockout system enables efficient generation of GPS2-deficient cell models for investigation of GPS2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPS2 exon(s) critical for GPS2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPS2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPS2 CRISPR/Cas9 KO Plasmid (h) and GPS2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPS2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPS2 HDR Plasmid (h) and GPS2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPS2 homology arms to support homology-directed repair at defined GPS2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.