GPRC5D Double Nickase Plasmid (h): sc-403114-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPRC5D Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPRC5D Double Nickase Plasmid (h) and GPRC5D Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPRC5D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPRC5D Antibody (E-2): sc-515733
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPRC5D Double Nickase Plasmid (h) | sc-403114-NIC | 20 µg | $410.00 | |||
GPRC5D Double Nickase Plasmid (h2) | sc-403114-NIC-2 | 20 µg | $410.00 |
GPRC5D (G protein-coupled receptor class C group 5 member D) is a human orphan GPCR with prominent expression in differentiated epithelial compartments, where it is linked to regulation of cell state, stress responses, and context-dependent signaling programs. As a membrane receptor-like protein, GPRC5D is positioned to modulate downstream pathways that integrate with MAPK and other GPCR-associated signaling networks, influencing transcriptional outputs and cellular adaptation. Altered GPRC5D expression has been reported in several disease contexts, including malignancies, making it a useful node for studying lineage markers, receptor-mediated signaling, and tumor-associated gene regulation. Its restricted expression patterns also support investigations of cell-type specificity and surface protein biology in human models.
GPRC5D Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPRC5D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPRC5D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPRC5D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPRC5D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.