GPRC5B Antibody (A-8): sc-518246

GPRC5B Antibody (A-8) is a mouse monoclonal IgG3 kappa light chain antibody that detects an epitope mapping between amino acids 348-377 of GPRC5B of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GPRC5B antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GPRC5B, or G protein-coupled receptor family C group 5 member B, is a 403 amino acid protein encoded by the human GPRC5B gene and is classified as an orphan receptor within the G protein-coupled receptor family. GPRC5B plays a crucial role in mediating cellular responses to retinoic acid, which is vital for various physiological processes, including cell differentiation and growth. GPRC5B is predominantly expressed in the kidney, pancreas, and testis, with moderate levels found in the brain, heart, prostate, small intestine, and spleen, highlighting potential significance in both endocrine and exocrine functions. The unique structure of GPRC5B, characterized by seven transmembrane domains, allows effective transduction of extracellular signals through heterotrimeric G proteins, thereby influencing critical signaling pathways. GPRC5B′s interaction with retinoid signaling pathways suggests importance in linking metabolic processes with cellular signaling, making anti-GPRC5B antibody (A-8) an indispensable tool for studying these intricate roles in retinoid signaling and broader implications in cellular communication and physiological regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GPRC5B Antibody (A-8) References:

1. Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain.  |  Bräuner-Osborne, H. and Krogsgaard-Larsen, P. 2000. Genomics. 65: 121-8. PMID: 10783259
2. Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C).  |  Robbins, MJ., et al. 2000. Genomics. 67: 8-18. PMID: 10945465
3. Identification of G protein-coupled receptor genes from the human genome sequence.  |  Takeda, S., et al. 2002. FEBS Lett. 520: 97-101. PMID: 12044878
4. Localisation of the GPRC5B receptor in the rat brain and spinal cord.  |  Robbins, MJ., et al. 2002. Brain Res Mol Brain Res. 106: 136-44. PMID: 12393273
5. The RAIG family member, GPRC5D, is associated with hard-keratinized structures.  |  Inoue, S., et al. 2004. J Invest Dermatol. 122: 565-73. PMID: 15086536
6. Changes in expression and localization of GPRC5B and RARalpha in the placenta and yolk sac during middle to late gestation in mice.  |  Imanishi, S., et al. 2007. J Reprod Dev. 53: 1131-6. PMID: 17652913
7. GPRC5b Modulates Inflammatory Response in Glomerular Diseases via NF-κB Pathway.  |  Zambrano, S., et al. 2019. J Am Soc Nephrol. 30: 1573-1586. PMID: 31285284
8. GPRC5B promotes collagen production in myofibroblasts.  |  Takizawa, N., et al. 2021. Biochem Biophys Res Commun. 561: 180-186. PMID: 34023784
9. The orphan receptor GPRC5B activates pro-inflammatory signaling in the vascular wall via Fyn and NFκB.  |  Freundt, GV., et al. 2022. Biochem Biophys Res Commun. 592: 60-66. PMID: 35033869
10. Orphan G-Protein Coupled Receptor GPRC5B Is Critical for Lymphatic Development.  |  Xu, W., et al. 2022. Int J Mol Sci. 23: PMID: 35628521
11. Aquaporin-4 and GPRC5B: old and new players in controlling brain oedema.  |  Passchier, EMJ., et al. 2023. Brain. 146: 3444-3454. PMID: 37143309
12. GPRC5B protects osteoarthritis by regulation of autophagy signaling.  |  He, L., et al. 2023. Acta Pharm Sin B. 13: 2976-2989. PMID: 37521864

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GPRC5B Antibody (A-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-GPRC5B Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GPRC5B Antibody (A-8) Specifications

Alternate Product Name: GPRC5B

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 348-377

Epitope Target: GPRC5B

Epitope Species: human

Product Reactivity/Detection: GPRC5B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for GPRC5B Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin