Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPR84 CRISPR/Cas9 KO Plasmid (h): sc-401953
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR84 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR84 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPR84 Antibody (1D9): sc-293447
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR84 CRISPR/Cas9 KO Plasmid (h) | sc-401953 | 20 µg | $397.00 |
Overview
GPR84 encodes a G protein-coupled receptor that is activated by medium-chain fatty acids and is enriched in myeloid lineage cells, linking lipid sensing to innate immune signaling. Upon stimulation, GPR84 engages GPCR-mediated pathways that modulate second messenger signaling and downstream transcriptional programs controlling cytokine production, chemotaxis, and macrophage activation. This receptor has been studied in the context of inflammation-associated metabolic remodeling and immune cell polarization within tissue microenvironments. Altered GPR84 activity has been reported across inflammatory and fibrotic disease models, supporting its use as a mechanistic node for interrogating immunometabolic pathways.
GPR84 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPR84 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPR84 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPR84 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR84 protein expression.
This CRISPR knockout system enables efficient generation of GPR84-deficient cell models for investigation of GPR84 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPR84 exon(s) critical for GPR84 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPR84 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR84 CRISPR/Cas9 KO Plasmid (h) and GPR84 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPR84 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR84 HDR Plasmid (h) and GPR84 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPR84 homology arms to support homology-directed repair at defined GPR84 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.