GPR41 CRISPR/Cas9 KO Plasmid (h): sc-402190
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR41 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR41 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR41 CRISPR/Cas9 KO Plasmid (h) | sc-402190 | 20 µg | $397.00 |
Overview
FFAR3 encodes the G protein-coupled receptor GPR41, a cell-surface sensor for short-chain fatty acids such as acetate, propionate, and butyrate produced by microbial fermentation. Upon ligand engagement, GPR41 primarily couples to Gi/o signaling to reduce cAMP and modulate downstream pathways that influence cellular metabolism, secretory programs, and inflammatory tone in a context-dependent manner. FFAR3 activity has been linked to host–microbiome communication, regulation of energy balance, and neuroendocrine and immune signaling networks. Altered FFAR3/GPR41 signaling is studied in metabolic and inflammatory disease biology, where it may affect adiposity, insulin sensitivity, and cytokine-associated responses without implying clinical outcomes.
GPR41 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FFAR3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FFAR3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FFAR3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR41 protein expression.
This CRISPR knockout system enables efficient generation of FFAR3-deficient cell models for investigation of GPR41 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FFAR3 exon(s) critical for GPR41 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FFAR3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR41 CRISPR/Cas9 KO Plasmid (h) and GPR41 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FFAR3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR41 HDR Plasmid (h) and GPR41 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FFAR3 homology arms to support homology-directed repair at defined FFAR3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.