GPR40 CRISPR/Cas9 KO Plasmid (h): sc-401434
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR40 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR40 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR40 CRISPR/Cas9 KO Plasmid (h) | sc-401434 | 20 µg | $397.00 |
Overview
FFAR1 encodes the free fatty acid receptor 1 (GPR40), a G protein-coupled receptor activated by medium- and long-chain fatty acids that couples primarily to Gq/11 signaling. Upon activation, GPR40 promotes phospholipase C activity, intracellular calcium mobilization, and downstream kinase pathways that regulate stimulus-secretion coupling and cellular metabolic responses. In pancreatic islets and related metabolic tissues, this receptor integrates lipid-derived cues with nutrient sensing to influence insulin secretory dynamics and broader glucose homeostasis networks. Dysregulated FFAR1/GPR40 signaling has been linked to metabolic disease-associated phenotypes, including altered beta-cell function and lipid-driven inflammatory signaling, supporting its relevance for mechanistic studies in diabetes and obesity research.
GPR40 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FFAR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FFAR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FFAR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR40 protein expression.
This CRISPR knockout system enables efficient generation of FFAR1-deficient cell models for investigation of GPR40 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FFAR1 exon(s) critical for GPR40 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FFAR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR40 CRISPR/Cas9 KO Plasmid (h) and GPR40 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FFAR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR40 HDR Plasmid (h) and GPR40 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FFAR1 homology arms to support homology-directed repair at defined FFAR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.