GPR38 CRISPR/Cas9 KO Plasmid (h): sc-406289
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR38 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR38 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR38 CRISPR/Cas9 KO Plasmid (h) | sc-406289 | 20 µg | $397.00 |
Overview
Motilin receptor (MLNR), also known as GPR38, is a class A G protein-coupled receptor that binds the peptide hormone motilin to coordinate gastrointestinal interdigestive motility and regulate smooth muscle contractility. Upon activation, GPR38 engages heterotrimeric G proteins to influence second-messenger signaling, including calcium mobilization and downstream kinase pathways that shape excitability in enteric and gastric circuits. MLNR expression and signaling are used to interrogate mechanisms controlling migrating motor complexes, gastric emptying, and neuroendocrine communication within the gut. Dysregulated motilin receptor activity has been investigated in the context of functional gastrointestinal disorders and altered gut motility phenotypes, making MLNR a relevant target for pathway-centric studies in human cellular models.
GPR38 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MLNR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MLNR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MLNR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR38 protein expression.
This CRISPR knockout system enables efficient generation of MLNR-deficient cell models for investigation of GPR38 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MLNR exon(s) critical for GPR38 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MLNR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR38 CRISPR/Cas9 KO Plasmid (h) and GPR38 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MLNR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR38 HDR Plasmid (h) and GPR38 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MLNR homology arms to support homology-directed repair at defined MLNR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.