Date published: 2026-5-29

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GPR3 Antibody (18-L): sc-100300

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Datasheets
  • GPR3 Antibody (18-L) is a mouse monoclonal IgG2a κ provided at 100 µg/ml
  • raised against recombinant GPR3 of human origin
  • recommended for detection of GPR3 of human origin by WB, IP, IF, IHC(P) and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GPR3 Antibody (18-L). This work is in progress.
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    GPR3 Antibody (18-L) is a mouse monoclonal IgG2a antibody that detects G protein-coupled receptor 3 (GPR3) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GPR3 is a 330 amino acid member of the G protein-coupled receptor 1 family, primarily located in the cellular membrane of cells, with significant expression in the central nervous system. GPR3 plays a crucial role in the regulation of cyclic adenosine monophosphate (cAMP) levels, which is vital for various cellular processes, including neurite outgrowth and myelin inhibition. GPR3′s ability to stimulate cAMP production is particularly important in oocytes, where GPR3 can halt meiosis and prevent progesterone-induced meiotic maturation, thereby influencing reproductive processes. Notably, mice lacking GPR3 can reproduce but exhibit a lack of control over oocyte maturation, leading to nondeveloping early embryos and fragmented oocytes as they age. Anti-GPR3 antibody (18-L) is an essential tool for researchers studying the intricate roles of GPR3 in cellular signaling and reproductive biology.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    GPR3 Antibody (18-L)

    sc-100300
    100 µg/ml
    $339.00

    Can sc-100300: GPR3 (18-L) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Professor Griffin
    Thank you for your question. Yes, sc-100300: GPR3 (18-L) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 2 out of 5 by from 感想と質問・一過的に発現させたGPR3はWBで検出できたが、内在性のGPR3は検出できなかった。SDS-PAGEのサンプルは、ボイルしていますか? ・この抗体(18-L)は、Abnova社のGPR3 monoclonal antibody (M01), clone 3B4-G3 と同じクローン抗体ですか?(同じWB, IHCデータが使用されています)
    Date published: 2023-06-20
    Rated 4 out of 5 by from Clear band with good signal when used onClear band with good signal when used on Jurkat whole cell lysate. -SCBT QC
    Date published: 2015-04-30
    Rated 5 out of 5 by from Clean signal obtained in WB on nonClean signal obtained in WB on non-transfected 293T, human GPR3 transfected 293T and Jurkat whole cell lysates with some PTMs causing double band. -SCBT QC
    Date published: 2015-03-26
    Rated 4 out of 5 by from Produced robust immunoperoxidase membraneProduced robust immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human transitional cell carcinoma tissue. -SCBT QC
    Date published: 2015-03-08
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    GPR3 Antibody (18-L) is rated 3.8 out of 5 by 4.
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