GPR19 CRISPR/Cas9 KO Plasmid (h): sc-407000
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR19 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR19 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR19 CRISPR/Cas9 KO Plasmid (h) | sc-407000 | 20 µg | $397.00 |
Overview
GPR19 encodes an orphan G protein-coupled receptor that is predominantly localized to the plasma membrane and is thought to couple to heterotrimeric G proteins to influence second-messenger signaling and downstream transcriptional programs. Although its endogenous ligand remains incompletely defined, GPR19 activity has been linked to regulation of cellular excitability, metabolic signaling, and context-dependent control of proliferation and differentiation. GPCR-mediated pathways associated with GPR19 can intersect with cAMP/PKA, MAPK/ERK, and calcium-dependent signaling, shaping cell-state decisions and stress responses. Altered GPCR signaling landscapes, including dysregulated expression of orphan receptors such as GPR19, are investigated in multifactorial conditions spanning neurobiology, inflammation, and cancer-related phenotypes, supporting its relevance for mechanistic studies.
GPR19 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPR19 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPR19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPR19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR19 protein expression.
This CRISPR knockout system enables efficient generation of GPR19-deficient cell models for investigation of GPR19 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPR19 exon(s) critical for GPR19 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPR19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR19 CRISPR/Cas9 KO Plasmid (h) and GPR19 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPR19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR19 HDR Plasmid (h) and GPR19 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPR19 homology arms to support homology-directed repair at defined GPR19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.