GPR171 CRISPR/Cas9 KO Plasmid (h): sc-412047
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR171 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR171 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR171 CRISPR/Cas9 KO Plasmid (h) | sc-412047 | 20 µg | $397.00 |
Overview
GPR171 encodes an orphan class A (rhodopsin-like) G protein-coupled receptor that is predominantly coupled to inhibitory signaling and is implicated in modulating intracellular cAMP and downstream transcriptional programs. It is expressed in immune and neural-associated contexts, where it has been linked to regulation of cell signaling tone, neuromodulatory circuits, and inflammatory responsiveness. Reported interactions with the neuropeptide BigLEN suggest a role in peptide-mediated GPCR signaling and network-level control of cellular activation states. Altered GPCR signaling involving GPR171 has been explored in relation to neurobehavioral phenotypes and immune regulation, supporting its relevance for pathway-level studies of receptor-mediated signal transduction.
GPR171 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPR171 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPR171 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPR171 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR171 protein expression.
This CRISPR knockout system enables efficient generation of GPR171-deficient cell models for investigation of GPR171 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPR171 exon(s) critical for GPR171 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPR171 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR171 CRISPR/Cas9 KO Plasmid (h) and GPR171 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPR171 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR171 HDR Plasmid (h) and GPR171 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPR171 homology arms to support homology-directed repair at defined GPR171 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.