GPR142 CRISPR/Cas9 KO Plasmid (h): sc-415656
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR142 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR142 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR142 CRISPR/Cas9 KO Plasmid (h) | sc-415656 | 20 µg | $397.00 |
Overview
GPR142 encodes G protein-coupled receptor 142, an orphan/class A GPCR enriched in metabolically relevant tissues and linked to nutrient sensing. Upon activation, GPR142 couples to heterotrimeric G proteins to regulate second-messenger signaling and calcium-dependent pathways that influence hormone secretion and broader metabolic homeostasis. Transcriptional and genetic studies have associated GPR142 with endocrine and metabolic phenotypes, including pathways relevant to glucose regulation and pancreatic islet function. As a cell-surface receptor, it provides a tractable node for dissecting GPCR signaling, ligand discovery, and receptor-driven transcriptional programs.
GPR142 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPR142 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPR142 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPR142 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR142 protein expression.
This CRISPR knockout system enables efficient generation of GPR142-deficient cell models for investigation of GPR142 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPR142 exon(s) critical for GPR142 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPR142 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR142 CRISPR/Cas9 KO Plasmid (h) and GPR142 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPR142 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR142 HDR Plasmid (h) and GPR142 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPR142 homology arms to support homology-directed repair at defined GPR142 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.