GPR119 CRISPR/Cas9 KO Plasmid (m): sc-433515
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR119 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR119 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR119 CRISPR/Cas9 KO Plasmid (m) | sc-433515 | 20 µg | $397.00 |
Overview
Gpr119 encodes GPR119, a class A GPCR enriched in pancreatic islet cells and intestinal enteroendocrine populations, where it couples primarily to Gs signaling to elevate intracellular cAMP. This pathway supports nutrient-sensing responses that influence incretin release, insulin secretion dynamics, and broader regulation of glucose and lipid homeostasis. In immune and metabolic tissues, GPR119-dependent signaling has been linked to modulation of cellular energy balance and hormone-driven cross-talk between gut and pancreas. Dysregulation of GPR119-associated networks is therefore studied in the context of metabolic phenotypes, including obesity- and diabetes-relevant pathways, as well as endocrine cell function.
GPR119 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gpr119 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gpr119 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gpr119 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR119 protein expression.
This CRISPR knockout system enables efficient generation of Gpr119-deficient cell models for investigation of GPR119 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gpr119 exon(s) critical for GPR119 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gpr119 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR119 CRISPR/Cas9 KO Plasmid (m) and GPR119 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gpr119 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR119 HDR Plasmid (m) and GPR119 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gpr119 homology arms to support homology-directed repair at defined Gpr119 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.